Identification of glycosylated regions in pneumococcal PspA conjugated to serotype 6B capsular polysaccharide

Autor(es)

Barazzone, Giovana Cappio ^[1] ; Pinto, Vittoria ^[2] ; Donnarumma, Danilo ^[2] ; Tanizaki, Martha Massako ^[1] ; Norais, Nathalie ^[2] ; Berti, Francesco ^[2]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil

^[2] Novartis Vaccines & Diagnost, Siena - Italy

• Tipo de documento: Artigo Científico
• Fonte: GLYCOCONJUGATE JOURNAL; v. 31, n. 3, p. 259-269, APR 2014.
• Citações Web of Science: 2

Resumo

Conjugate vaccines are being widely used since their introduction. Nowadays the interest in these vaccines is still growing and new antigens and conjugate chemistry are being studied and developed. Pneumococcal surface protein A (PspA) is one of the most studied pneumococcal antigens and is an important vaccine candidate. One approach to broaden the conjugate vaccine coverage could be the conjugation of the polysaccharide to a pneumococcal protein such as PspA. Previous results have shown that conjugated recombinant fragment of PspA (rPspA) not only maintained but also in some conjugates improved the induction of protective antibodies raised against the protein carrier. We describe here a characterization study to identify the domains of Streptococcus pneumoniae recombinant PspA (rPspA), from family 1 clade 1 and family 2 clade 3, involved in the conjugation with serotype 6B capsular polysaccharide. (AU)

• Processo FAPESP: 12/13015-3 - Identificação de sítios de glicosilação em conjugados de polissacarídeo capsular de Streptococcus pneumoniae-PspA por espectrometria de massas
• Beneficiário: Giovana Cappio Barazzone
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 08/05207-4 - Vacina conjugada anti-pneumocócica: estudos sobre a viabilidade de uma vacina polissacarídeo capsular - PSPA
• Beneficiário: Martha Massako Tanizaki
• Modalidade de apoio: Auxílio à Pesquisa - Temático