Identification of glycosylated regions in pneumococcal PspA conjugated to serotype 6B capsular polysaccharide

Author(s)

Barazzone, Giovana Cappio ^[1] ; Pinto, Vittoria ^[2] ; Donnarumma, Danilo ^[2] ; Tanizaki, Martha Massako ^[1] ; Norais, Nathalie ^[2] ; Berti, Francesco ^[2]

Affiliation

^[1] Inst Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil

^[2] Novartis Vaccines & Diagnost, Siena - Italy

• Document type: Journal article
• Source: GLYCOCONJUGATE JOURNAL; v. 31, n. 3, p. 259-269, APR 2014.
• Web of Science Citations: 2

Abstract

Conjugate vaccines are being widely used since their introduction. Nowadays the interest in these vaccines is still growing and new antigens and conjugate chemistry are being studied and developed. Pneumococcal surface protein A (PspA) is one of the most studied pneumococcal antigens and is an important vaccine candidate. One approach to broaden the conjugate vaccine coverage could be the conjugation of the polysaccharide to a pneumococcal protein such as PspA. Previous results have shown that conjugated recombinant fragment of PspA (rPspA) not only maintained but also in some conjugates improved the induction of protective antibodies raised against the protein carrier. We describe here a characterization study to identify the domains of Streptococcus pneumoniae recombinant PspA (rPspA), from family 1 clade 1 and family 2 clade 3, involved in the conjugation with serotype 6B capsular polysaccharide. (AU)

• FAPESP's process: 12/13015-3 - Identification of glycosylation sites of S. pneumoniae capsular polysaccharide-PspA conjugates by mass spectrometry
• Grantee: Giovana Cappio Barazzone
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 08/05207-4 - Pneumococcal conjugate vaccine: capsular polysaccharide - pneumococcal surface protein A
• Grantee: Martha Massako Tanizaki
• Support Opportunities: Research Projects - Thematic Grants