Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum

Autor(es)

Pinheiro, Fabio ^{[1, 2]} ; Palma-Silva, Clarisse ^[3] ; de Barros, Fabio ^[4] ; Cozzolino, Salvatore ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bot, Inst Biociencias, BR-05508900 Sao Paulo - Brazil

^[2] Univ Naples Federico 2, Dipartimento Biol Strutturale & Funzionale, Complesso Univ Monte S Angelo, Naples - Italy

^[3] Univ Fed Rio de Janeiro, Dept Bot, Inst Biol, Rio De Janeiro - Brazil

^[4] Inst Bot, Secao Orquidario Estado, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 32, n. 2, p. 337-339, 2009.
• Citações Web of Science: 15

Resumo

In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus. (AU)

• Processo FAPESP: 06/54189-3 - Implicações taxonômicas da estrutura genética de populações naturais de E. cinnabarinum Salzm., E. denticulatum Barb. Rodr. e E. fulgens Brongn.
• Beneficiário: Fábio de Barros
• Modalidade de apoio: Auxílio à Pesquisa - Regular