Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)

Autor(es)

Moraes de Castro e Souza, Andiara Silos ^[1] ; Mino, Carolina Isabel ^[1] ; Del Lama, Silvia Nassif ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-15365905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 35, n. 1, p. 74-80, 2012.
• Citações Web of Science: 3

Resumo

We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was contained within populations. Structure analysis suggested a single population, and F ST value showed weak genetic structuring (F ST = 0.009, p = 0.05). The two populations are apparently connected through gene-flow. The panel of six microsatellites optimized here was sufficiently informative for characterizing the genetic diversity and structure in these natural populations of the white-faced ibis. The information generated could be useful in future studies of genetic diversity, relatedness and the mating system in Plegadis chihi and related species. (AU)

• Processo FAPESP: 10/50406-5 - Colonização do continente americano pelas populações africanas de garça-vaqueira (Bubulcus ibis): a genética desvendando o processo de bioinvasão
• Beneficiário: Silvia Nassif Del Lama
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/15205-8 - Genética de populações de Ciconiiformes (Aves) em áreas úmidas brasileiras
• Beneficiário: Silvia Nassif Del Lama
• Modalidade de apoio: Auxílio à Pesquisa - Regular