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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)

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Author(s):
Moraes de Castro e Souza, Andiara Silos [1] ; Mino, Carolina Isabel [1] ; Del Lama, Silvia Nassif [1]
Total Authors: 3
Affiliation:
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-15365905 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 35, n. 1, p. 74-80, 2012.
Web of Science Citations: 3
Abstract

We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was contained within populations. Structure analysis suggested a single population, and F ST value showed weak genetic structuring (F ST = 0.009, p = 0.05). The two populations are apparently connected through gene-flow. The panel of six microsatellites optimized here was sufficiently informative for characterizing the genetic diversity and structure in these natural populations of the white-faced ibis. The information generated could be useful in future studies of genetic diversity, relatedness and the mating system in Plegadis chihi and related species. (AU)

FAPESP's process: 10/50406-5 - Colonization of the American Continent by African populations of cattle egret (Bubulcus ibis) and its parasites (Plasmodium and Haemoproteus): genetics revealing the bioinvasion process
Grantee:Silvia Nassif Del Lama
Support Opportunities: Regular Research Grants