Microsatellites and SNPs identification in Cattle Egret (Bubulcus ibis, Ardeidae, Aves) and its application in familial and population studies

• Grant number: 16/01673-7
• Support type: Regular Research Grants
• Duration: July 01, 2016 - June 30, 2018
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal researcher: Silvia Nassif Del Lama
• Grantee: Silvia Nassif Del Lama
• Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Assoc. researchers: Carolina Isabel Miño

Abstract

This proposal aims to isolate new genetic markers, microsatellites and SNPs (Single Nucleotide Polymorphisms) and apply them in families and populations studies of the Cattle Egret (Bubulcus ibis). The panel of polymorphic microsatellite will be expanded with the use of data generated by Next Generation Sequencing (NGS). SNPs will be identified by GBS method (Genotyping By Sequencing). Genotypes in these new microsatellite loci will be identified in all members of the 30 families, parents and offspring, and kin will be verified by Mendelian and by Maximum Likelihood analysis. Proposal will confirm or not the occurrence of alternative behaviors to monogamy (Fertilization Extra-Pair and Parasitism Nest Intraspecific). Genotypes in microsatellite loci will be determined in four African populations and in a representative of Brazilian population to investigate the genetic diversity level of these populations and if they differ genetically. Data for SNPs will be used in the same populations to determine the level of diversity and genetic structuring among them. The results of the populational analysis with both types of molecular markers (SNPs and microsatellites) will be compared and it is expected to obtain a much higher degree of discrimination populational by SNPs than by microsatellites. Results will show if there is a degree of differentiation and isolation between populations of the two continents, confirming the result obtained previously and discarding that is an artifact, or they will point that there is a continuous bird inflow from Africa to Brazil. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILLAR COUTO, CYNTHIA M.; CUMMING, GRAEME S.; LACORTE, GUSTAVO A.; CONGRAINS, CARLOS; IZBICKI, RAFAEL; BRAGA, ERIKA MARTINS; ROCHA, CRISTIANO D.; MORALEZ-SILVA, EMMANUEL; HENRY, DOMINIC A. W.; MANU, SHIIWUA A.; ABALAKA, JACINTA; REGALLA, AISSA; DA SILVA, ALFREDO SIMAO; DIOP, MOUSSA S.; DEL LAMA, SILVIA N. Avian haemosporidians in the cattle egret (Bubulcus ibis) from central-western and southern Africa: High diversity and prevalence. PLoS One, v. 14, n. 2 FEB 22 2019. Web of Science Citations: 0.

DA SILVA, FAGNER M.; MINO, CAROLINA I.; IZBICKI, RAFAEL; DEL LAMA, SILVIA N. Considerations for monitoring population trends of colonial waterbirds using the effective number of breeders and census estimates. ECOLOGY AND EVOLUTION, v. 8, n. 16, p. 8088-8101, AUG 2018. Web of Science Citations: 0.

CHAHAD-EHLERS, SAMIRA; FUSHITA, ANGELA TERUMI; LACORTE, GUSTAVO AUGUSTO; PEREIRA DE ASSIS, PAMELA CARLA; DEL LAMA, SILVIA NASSIF. Effects of habitat suitability for vectors, environmental factors and host characteristics on the spatial distribution of the diversity and prevalence of haemosporidians in waterbirds from three Brazilian wetlands. PARASITES & VECTORS, v. 11, MAY 2 2018. Web of Science Citations: 2.

MORALEZ-SILVA, EMMANUEL; DEL LAMA, SILVIA NASSIF. Multiple Females Laying in Cattle Egret (&ITBubulcus ibis&IT) Clutches: Behavioral and Genetic Evidence. WATERBIRDS, v. 40, n. 4, p. 403-409, DEC 2017. Web of Science Citations: 0.

MINO, CAROLINA ISABEL; DE SOUZA, ELAINE DANTAS; MORALEZ-SILVA, EMMANUEL; VALDES, TALITA ALVARENGA; CORTICO CORREA RODRIGUES, VERA LUCIA; DEL LAMA, SILVIA NASSIF. Use of noninvasive `bug-eggs' to enable comparative inferences on genetic mating system with and without parental information: A study in a cattle egret colony. PLoS One, v. 12, n. 8 AUG 30 2017. Web of Science Citations: 1.