| Texto completo | |
| Autor(es): |
Ferreira Albani, Silvia Maria
[1]
;
da Silva, Mateus Ribeiro
[2]
;
Takagi, Mickie
[3]
;
Cabrera-Crespo, Joaquin
[4]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Inst Butantan. Ctr Biotecnol
[2] Inst Butantan. Ctr Biotecnol
[3] Inst Butantan. Ctr Biotecnol
[4] Inst Butantan. Ctr Biotecnol
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Applied Biochemistry and Biotechnology; v. 167, n. 7, p. 2068-2075, AUG 2012. |
| Citações Web of Science: | 5 |
| Resumo | |
Capsular polysaccharide produced by Haemophilus influenzae b (Hib) is the main virulent agent and used as the antigen in the vaccine formulation. In this study, an improved process of polysaccharide purification was established based on tangential flow ultrafiltration using detergents (cocamidopropyl betaine and sodium deoxycholate), two selective ethanol precipitations steps, and extensive enzymatic hydrolysis as strategy. The relative purity (RP) related to protein and nucleic acids were 122 similar to 263 and 294 similar to 480, respectively, and compatible with the specifications established by the World Health Organization for Hib vaccine, RP a parts per thousand yenaEuro parts per thousand 100. These results make this process simple, cheaper, efficient, environmentally friendly, and prone to be scaled up. (AU) | |
| Processo FAPESP: | 07/50082-2 - Utilização de métodos cromatográficos e ultrafiltração tangencial na purificação de polissacarídeos vacinais de origem bacteriana |
| Beneficiário: | Joaquin Cabrera-Crespo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |