Improvement in the Purification Process of the Capsular Polysaccharide from Haemophilus influenzae Type b by Using Tangential Ultrafiltration and Diafiltration

Ferreira Albani, Silvia Maria; da Silva, Mateus Ribeiro; Takagi, Mickie; Cabrera-Crespo, Joaquin

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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improvement in the Purification Process of the Capsular Polysaccharide from Haemophilus influenzae Type b by Using Tangential Ultrafiltration and Diafiltration

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Author(s):	Ferreira Albani, Silvia Maria ^[1] ; da Silva, Mateus Ribeiro ^[2] ; Takagi, Mickie ^[3] ; Cabrera-Crespo, Joaquin ^[4] Total Authors: 4
Affiliation:	^[1] Inst Butantan. Ctr Biotecnol ^[2] Inst Butantan. Ctr Biotecnol ^[3] Inst Butantan. Ctr Biotecnol ^[4] Inst Butantan. Ctr Biotecnol Total Affiliations: 4
Document type:	Journal article
Source:	Applied Biochemistry and Biotechnology; v. 167, n. 7, p. 2068-2075, AUG 2012.
Web of Science Citations:	5
Abstract
Capsular polysaccharide produced by Haemophilus influenzae b (Hib) is the main virulent agent and used as the antigen in the vaccine formulation. In this study, an improved process of polysaccharide purification was established based on tangential flow ultrafiltration using detergents (cocamidopropyl betaine and sodium deoxycholate), two selective ethanol precipitations steps, and extensive enzymatic hydrolysis as strategy. The relative purity (RP) related to protein and nucleic acids were 122 similar to 263 and 294 similar to 480, respectively, and compatible with the specifications established by the World Health Organization for Hib vaccine, RP a parts per thousand yenaEuro parts per thousand 100. These results make this process simple, cheaper, efficient, environmentally friendly, and prone to be scaled up. (AU)

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