Sintered anorganic bone graft increases autocrine expression of VEGF, MMP-2 and MMP-9 during repair of critical-size bone defects

Autor(es)

Rocha, Caroline Andrade ^[1] ; Cestari, Tania Mary ^[1] ; Vidotti, Hugo Alberto ^[1] ; de Assis, Gerson Francisco ^[1] ; Garlet, Gustavo Pompermaier ^[1] ; Taga, Rumio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Molecular Histology; v. 45, n. 4, p. 447-461, AUG 2014.
• Citações Web of Science: 11

Resumo

This study aimed to evaluate morphometrically the bone formation and immunohistochemically the expression of vascular endothelial growth factor (VEGF) and metalloproteinase (MMP)-2 and -9 during the healing of critical-size defects treated with sintered anorganic bone (sAB). The 8-mm diameter full-thickness trephine defects created in the parietal bones of rats were filled with sAB (test group) or blood clot (CSD-control group). At 7, 14, 21, 30, 90 and 180 days postoperatively (n = 6/period) the volume of newly formed bone and total number of immunolabeled cells (Ntm) for each protein were determined. Bone formation was smaller and faster in the CSD-control group, stabilizing at 21 days (6.74 mm(3)). The peaks of VEGF, MMP-2 and MMP-9 occurred at 7 and 14 days in fibroblasts and osteoblasts, with mean reduction of 0.80 time at 21 days, keeping constant until 180 days. In the test group, sAB provided continuous bone formation between particles throughout all periods. The peak of MMP-2 was observed at 7-14 days in connective tissue cells and for VEGF and MMP-9 at 30 days in osteoblasts and osteocytes. Ntm for VEGF, MMP-2 and MMP-9 were in average, respectively, 3.70, 2.03 and 5.98 times higher than in the control group. At 180 days, newly formed bone (22.9 mm(3)) was 3.74 times greater in relation to control. The physical and chemical properties of sAB allow increased autocrine expression of VEGF, MMP-2 and MMP-9, favoring bone formation/remodeling with very good healing of cranial defects when compared to natural repair in the CSD-control. (AU)

• Processo FAPESP: 05/02310-0 - Cinetica celular e tecidual durante a regeneracao ossea em defeitos cranianos perenes em ratos tratados com osso inorganico poroso bovino.
• Beneficiário: Hugo Alberto Vidotti
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 05/02221-8 - Analise imunohistoquimica quantitativa da expressao de mmp-1, mmp-2, mmp-9 e do vegf durante a reparacao de defeito osseo de tamanho critico em calvaria de rato tratado com osso desproteinado poroso x...
• Beneficiário: Caroline Andrade Rocha
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica