Bauhinia forficata lectin (BfL) induces cell death and inhibits integrin-mediated adhesion on MCF7 human breast cancer cells

Autor(es)

Silva, Mariana C. C. ^[1] ; de Paula, Claudia A. A. ^[1] ; Ferreira, Joana G. ^[1] ; Paredes-Gamero, Edgar J. ^{[1, 2]} ; Vaz, Angela M. S. F. ^[3] ; Sampaio, Misako U. ^[1] ; Correia, Maria Tereza S. ^[4] ; Oliva, Maria Luiza V. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biofis, BR-04044020 Sao Paulo - Brazil

^[3] Inst Pesquisas Jardim Bot Rio de Janeiro, BR-22460030 Rio De Janeiro, RJ - Brazil

^[4] Univ Fed Pernambuco, Dept Bioquim, BR-50670910 Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS; v. 1840, n. 7, p. 2262-2271, JUL 2014.
• Citações Web of Science: 23

Resumo

Background: Plant lectins have attracted great interest in cancer studies due to their antitumor activities. These proteins or glycoproteins specifically and reversibly bind to different types of carbohydrates or glycoproteins. Breast cancer, which presents altered glycosylation of cell surface glycoproteins, is one of the most frequent malignant diseases in women. In this work, we describe the effect of the lectin Bauhinia forficata lectin (BfL), which was purified from B. forficata Link subsp.forficata seeds, on the MCF7 human breast cancer cellular line, investigating the mechanisms involved in its antiproliferative activity. Methods: MCF7 cells were treated with BfL Viability and adhesion alterations were evaluated using flow cytometry and western blotting. Results: BfL. inhibited the viability of the MCF7 cell line but was ineffective on MDA-MB-231 and MCF 10A cells. It inhibits MCF7 adhesion on laminin, collagen I and fibronectin, decreases alpha(1), alpha(6) and beta(1) integrin subunit expression, and increases alpha(5) subunit expression. BfL triggers necrosis and secondary necrosis, with caspase-9 inhibition. It also causes deoxyribonucleic add (DNA) fragmentation, which leads to cell cycle arrest in the G2/M phase and a decrease in the expression of the regulatory proteins pRb and p21. Conclusion: BfL shows selective cytotoxic effect and adhesion inhibition on MCF7 breast cancer cells. General significance: Cell death induction and inhibition of cell adhesion may contribute to understanding the action of lectins in breast cancer. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/06366-4 - Investigação dos mecanismos de ação da lectina BfL (Bauhinia forficata) em linhagens tumorais: relação entre estrutura e especificidade.
• Beneficiário: Mariana Cristina Cabral Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/58929-4 - Purificacao, caracterizacao bioquimica e fisiologica de lectinas de plantas do genero bauhinia e sua acao sobre linhagens celulares tumorigenicas.
• Beneficiário: Mariana Cristina Cabral Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/53766-5 - Proteínas de origem vegetal com seletividade para inibição de enzimas de mamíferos e seu papel como agente anti-inflamatório, antitrombótico, antidiabético e antitumoral
• Beneficiário: Maria Luiza Vilela Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Temático