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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bauhinia forficata lectin (BfL) induces cell death and inhibits integrin-mediated adhesion on MCF7 human breast cancer cells

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Author(s):
Silva, Mariana C. C. [1] ; de Paula, Claudia A. A. [1] ; Ferreira, Joana G. [1] ; Paredes-Gamero, Edgar J. [1, 2] ; Vaz, Angela M. S. F. [3] ; Sampaio, Misako U. [1] ; Correia, Maria Tereza S. [4] ; Oliva, Maria Luiza V. [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Biofis, BR-04044020 Sao Paulo - Brazil
[3] Inst Pesquisas Jardim Bot Rio de Janeiro, BR-22460030 Rio De Janeiro, RJ - Brazil
[4] Univ Fed Pernambuco, Dept Bioquim, BR-50670910 Recife, PE - Brazil
Total Affiliations: 4
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS; v. 1840, n. 7, p. 2262-2271, JUL 2014.
Web of Science Citations: 23
Abstract

Background: Plant lectins have attracted great interest in cancer studies due to their antitumor activities. These proteins or glycoproteins specifically and reversibly bind to different types of carbohydrates or glycoproteins. Breast cancer, which presents altered glycosylation of cell surface glycoproteins, is one of the most frequent malignant diseases in women. In this work, we describe the effect of the lectin Bauhinia forficata lectin (BfL), which was purified from B. forficata Link subsp.forficata seeds, on the MCF7 human breast cancer cellular line, investigating the mechanisms involved in its antiproliferative activity. Methods: MCF7 cells were treated with BfL Viability and adhesion alterations were evaluated using flow cytometry and western blotting. Results: BfL. inhibited the viability of the MCF7 cell line but was ineffective on MDA-MB-231 and MCF 10A cells. It inhibits MCF7 adhesion on laminin, collagen I and fibronectin, decreases alpha(1), alpha(6) and beta(1) integrin subunit expression, and increases alpha(5) subunit expression. BfL triggers necrosis and secondary necrosis, with caspase-9 inhibition. It also causes deoxyribonucleic add (DNA) fragmentation, which leads to cell cycle arrest in the G2/M phase and a decrease in the expression of the regulatory proteins pRb and p21. Conclusion: BfL shows selective cytotoxic effect and adhesion inhibition on MCF7 breast cancer cells. General significance: Cell death induction and inhibition of cell adhesion may contribute to understanding the action of lectins in breast cancer. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/06366-4 - Investigation of mechanisms of action of the lectin BfL (Bauhinia forficata) in tumor cell lines: relation between structure and specificity.
Grantee:Mariana Cristina Cabral Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 09/53766-5 - Proteins from plant source with selectivity for inhibition of mammalian enzymes and their role as an anti-inflammatory, antithrombotic, anti-diabetic and anti-tumor agent
Grantee:Maria Luiza Vilela Oliva
Support Opportunities: Research Projects - Thematic Grants