Her2p molecular modeling, mutant analysis and intramitochondrial localization

Autor(es)

Ferreira-Junior, Jose Ribamar ^[1] ; Bleicher, Lucas ^[2] ; Barros, Mario H. ^[3]

Afiliação do(s) autor(es)

^[1] Escola Artes Ciencias & Humanidades USP. Sao Paulo

^[2] Univ Fed Minas Gerais. Inst Ciencias Biol

^[3] Univ Sao Paulo. Dept Microbiol

• Tipo de documento: Artigo Científico
• Fonte: Fungal Genetics and Biology; v. 60, n. SI, p. 133-139, NOV 2013.
• Citações Web of Science: 1

Resumo

Bacterial GatCAB amidotransferases are responsible for the transamidation of mischarged glutamyl-tRNA(Gln) into glutaminyl-tRNA(Gln). Mitochondria matrix also has a multienzymatic complex necessary for the transamidation of glutamyl-tRNA(Gln). Gtfl1p, Her2p and Pet112p are the constituents of mitochondrial GatFAB amidotransferase complex. Her2p is subunit A of GatFAB complex, while Gtf1p is subunit F, a connector protein between Pet112p (subunit B) and Her2p. Here we evaluate through molecular modeling and amino acid correlation analysis the HER2 protein family. Localization studies indicated that Her2p is predominantly localized in the mitochondrial outer membrane, but it is also located in the mitochondrial matrix where together with Pet112p and Gtf1p constitutes the GatFAB complex. Finally, HER2 random mutagenesis unveiled important residues that provide thermo stability for the complex and are differently suppressed by overexpression of GTF1 or PET112. For instance, her2/ts11 mutant showed its fermentative growth impaired, and poorly rescued by GTF1 indicating that Her2p unknown function in the mitochondria outer membrane affects cell viability. (C) 2013 Elspvier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/07366-5 - Estudo de proteínas mitocondriais de função desconhecida e suas consequências na viabilidade celular
• Beneficiário: Mario Henrique de Barros
• Modalidade de apoio: Auxílio à Pesquisa - Regular