Autor(es)

Santos, Fabio Martinez ^{[1, 2, 3, 4]} ; Grecco, Leandro Henrique ^[2] ; Pereira, Marcelo Gomes ^[3] ; Oliveira, Mara Evany ^[1] ; Rocha, Priscila Abreu ^[1] ; Silva, Joyce Teixeira ^[1] ; Martins, Daniel Oliveira ^[1] ; Miyabara, Elen Haruka ^[3] ; Chacur, Marucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Funct Neuroanat Pain, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Butantan Inst, Special Lab Pain & Signaling, BR-05503900 Butanta, SP - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Skeletal Muscle Plast, BR-05508000 Sao Paulo - Brazil

^[4] Univ Nove Julho, Dept Hlth Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Behavioral and Brain Functions; v. 10, MAY 13 2014.
• Citações Web of Science: 10

Resumo

Background: The neural mobilization (NM) technique is a noninvasive method that has been proven to be clinically effective in reducing pain; however, the molecular mechanisms involved remain poorly understood. The aim of this study was to analyze whether NM alters the expression of the mu-opioid receptor (MOR), the delta-opioid receptor (DOR) and the Kappa-opioid receptor (KOR) in the periaqueductal gray (PAG) and improves locomotion and muscle force after chronic constriction injury (CCI) in rats. Methods: The CCI was imposed on adult male rats followed by 10 sessions of NM every other day, starting 14 days after the CCI injury. At the end of the sessions, the PAG was analyzed using Western blot assays for opioid receptors. Locomotion was analyzed by the Sciatic functional index (SFI), and muscle force was analyzed by the BIOPAC system. Results: An improvement in locomotion was observed in animals treated with NM compared with injured animals. Animals treated with NM showed an increase in maximal tetanic force of the tibialis anterior muscle of 172% (p < 0.001) compared with the CCI group. We also observed a decrease of 53% (p < 0.001) and 23% (p < 0.05) in DOR and KOR levels, respectively, after CCI injury compared to those from naive animals and an increase of 17% (p < 0.05) in KOR expression only after NM treatment compared to naive animals. There were no significant changes in MOR expression in the PAG. Conclusion: These data provide evidence that a non-pharmacological NM technique facilitates pain relief by endogenous analgesic modulation. (AU)

• Processo FAPESP: 10/20026-6 - Uso do laser de baixa intensidade e os mecanismos celulares e moleculares no processo de reparação no sistema nervoso periférico
• Beneficiário: Daniel de Oliveira Martins
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 12/05840-4 - Mobilização Neural: Avaliação molecular e comportamental em ratos Wistar após indução de dor neuropática
• Beneficiário: Fabio Martinez dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/52520-0 - Efeito da suplementacao com leucina no processo de regeneracao muscular esqueletica.
• Beneficiário: Marcelo Gomes Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/24408-6 - Ressonância magnética e ativação neuronal supraespinal em modelo de dor neuropática crônica em ratos
• Beneficiário: Joyce Teixeira da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto