Auxin production by the plant trypanosomatid Phytomonas serpens and auxin homoeostasis in infected tomato fruits

Autor(es)

Ienne, Susan ^[1] ; Freschi, Luciano ^[2] ; Vidotto, Vanessa F. ^[1] ; de Souza, Tiago A. ^[3] ; Purgatto, Eduardo ^[4] ; Zingales, Bianca ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, CEFAP USP Ctr Facilidades Apoio Pesquisa USP, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut, NAPAN, Dept Alimentos & Nutr Expt, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology; v. 141, n. 10, p. 1299-1310, SEP 2014.
• Citações Web of Science: 1

Resumo

Previously we have characterized the complete gene encoding a pyruvate decarboxylase (PDC)/indolepyruvate decarboxylase (IPDC) of Phytomonas serpens, a trypanosomatid highly abundant in tomato fruits. Phylogenetic analyses indicated that the clade that contains the trypanosomatid protein behaves as a sister group of IPDCs of gamma-proteobacteria. Since IPDCs are key enzymes in the biosynthesis of the plant hormone indole-3-acetic acid (IAA), the ability for IAA production by P. serpens was investigated. Similar to many microorganisms, the production of IAA and related indolic compounds, quantified by high performance liquid chromatography, increased in P. serpens media in response to amounts of tryptophan. The auxin functionality was confirmed in the hypocotyl elongation assay. In tomato fruits inoculated with P. serpens the concentration of free IAA had no significant variation, whereas increased levels of IAA-amide and IAA-ester conjugates were observed. The data suggest that the auxin produced by the flagellate is converted to IAA conjugates, keeping unaltered the concentration of free IAA. Ethanol also accumulated in P. serpens-conditioned media, as the result of a PDC activity. In the article we discuss the hypothesis of the bifunctionality of P. serpens PDC/IPDC and provide a three-dimensional model of the enzyme. (AU)

• Processo FAPESP: 08/50209-5 - Phytomonas serpens: caracterizacao da piruvato/indolpiruvato descarboxilase e funcionalidade da putativa auxina produzida.
• Beneficiário: Susan Ienne da Silva Vançan
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/50957-1 - Subprojeto 1: tranportador ABC e resistência a benznidazol em Trypanosoma cruzi. Subprojeto 2: produção do fitormônio ácido indolil acético (AIA) em Phytomonas serpens
• Beneficiário: Bianca Silvana Zingales
• Modalidade de apoio: Auxílio à Pesquisa - Regular