Intrinsic Connections within the Pedunculopontine Tegmental Nucleus are Critical to the Elaboration of Post-Ictal Antinociception

Autor(es)

Mazzei-Silva, Elaine Cristina ^[1] ; de Oliveira, Rithiele Cristina ^[1] ; Garcia, Tayllon Dos Anjos ^[1] ; Falconi-Sobrinho, Luiz Luciano ^[1] ; Almada, Rafael Carvalho ^[1] ; Coimbra, Norberto Cysne ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FMRP USP, Ribeirao Preto Med Sch, Lab Neuroanat & Neuropsychobiol, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Synapse; v. 68, n. 8, p. 369-377, AUG 2014.
• Citações Web of Science: 9

Resumo

This study investigated the intrinsic connections of a key-structure of the endogenous pain inhibitory system, the pedunculopontine tegmental nucleus (PPTN), in post-ictal antinociceptive process through synaptic inactivation of the PPTN with cobalt chloride. Male Wistar rats (n = 6 or 7 per group), weighing 250-280 g, had the tail-flick baseline recorded and were submitted to a stereotaxic surgery for the introduction of a guide-cannula aiming at the PPTN. After 5 days of postoperative recovery, cobalt chloride (1 mM/0.2 mu L) or physiological saline (0.2 mu L) were microinjected into the PPTN and after 5 min, the tail-withdrawal latency was measured again at 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, and 120 min after seizures evoked by intraperitoneal injection of pentylenetetrazole (64 mg/kg). The synaptic inactivation of PPTN decreased the post-ictal antinociceptive phenomenon, suggesting the involvement of PPTN intrinsic connections in the modulation of pain, during tonic-clonic seizures. These results showed that the PPTN may be crucially involved in the neural network that organizes the post-ictal analgesia. (c) 2014 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 09/00668-6 - Estudo do envolvimento de vias colinérgicas que conectam o núcleo tegmentar pedúnculo-pontino a núcleos monoaminérgicos do sistema endógeno de inibição de dor na analgesia pós-ictal
• Beneficiário: Norberto Cysne Coimbra
• Modalidade de apoio: Auxílio à Pesquisa - Regular