Yolk Sac Mesenchymal Progenitor Cells from New World Mice (Necromys lasiurus) with Multipotent Differential Potential

Autor(es)

Favaron, Phelipe Oliveira ^[1] ; Mess, Andrea ^[1] ; Will, Sonia Elisabete ^[1] ; Maiorka, Paulo Cesar ^[1] ; de Oliveira, Moacir Franco ^[2] ; Miglino, Maria Angelica ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Sao Paulo - Brazil

^[2] Univ Fed Rural Semi Arido, Dept Anim Sci, Mossoro, RN - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 6 JUN 11 2014.
• Citações Web of Science: 7

Resumo

Fetal membranes are abundant, ethically acceptable and readily accessible sources of stem cells. In particular, the yolk sac is a source of cell lineages that do not express MHCs and are mainly free from immunological incompatibles when transferred to a recipient. Although data are available especially for hematopoietic stem cells in mice and human, whereas other cell types and species are dramatically underrepresented. Here we studied the nature and differentiation potential of yolk sac derived mesenchymal stem cells from a New World mouse, Necromys lasiurus. Explants from mid-gestation were cultured in DMEM-High glucose medium with 10% defined fetal bovine serum. The cells were characterized by standard methods including immunophenotyping by fluorescence and flow cytometry, growth and differentiation potential and tumorigenicity assays. The first adherent cells were observed after 7 days of cell culture and included small, elongated fibroblast-like cells (92.13%) and large, round epithelial-like cells with centrally located nuclei (6.5%). Only the fibroblast-like cells survived the first passages. They were positive to markers for mesenchymal stem cells (Stro-1, CD90, CD105, CD73) and pluripotency (Oct3/4, Nanog) as well as precursors of hematopoietic stem cells (CD117). In differentiation assays, they were classified as a multipotent lineage, because they differentiated into osteogenic, adipogenic, and chondrogenic lineages and, finally, they did not develop tumors. In conclusion, mesenchymal progenitor cells with multipotent differentiation potential and sufficient growth and proliferation abilities were able to be obtained from Necromys yolk sacs, therefore, we inferred that these cells may be promising for a wide range of applications in regenerative medicine. (AU)

• Processo FAPESP: 09/53392-8 - Placentação em Necromys lasiurus (Rodentia, Cricetidae, Sigmodontinae): características da eritrofagocitose, transporte placentário, inversão e versatilidade vitelina
• Beneficiário: Phelipe Oliveira Favaron
• Modalidade de apoio: Bolsas no Brasil - Doutorado