Reduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in the presence of antioxidants

Autor(es)

Rocha-Frigoni, Nathalia A. S. ^[1] ; Leao, Beatriz C. S. ^[1] ; Nogueira, Eriklis ^[2] ; Accorsi, Monica F. ^[1] ; Mingoti, Gisele Z. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Anim Hlth, Sch Vet Med, BR-16050680 Aracatuba, SP - Brazil

^[2] Brazilian Agr Res Corp, EMBRAPA Pantanal, BR-79320900 Corumba, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 26, n. 6, p. 797-805, 2014.
• Citações Web of Science: 11

Resumo

The effects of intracellular (cysteine and beta-mercaptoethanol) and extracellular (catalase) antioxidant supplementation at different times during in vitro production (IVM and/or in vitro culture (IVC)) on bovine embryo development, intracellular reactive oxygen species (ROS) levels, apoptosis and re-expansion rates after a vitrification-thawing process were examined. Blastocyst frequencies were not affected by either antioxidant supplementation (40.5%56.4%) or the timing of supplementation (41.7%-55.4%) compared with control (48.7%; P>0.05). Similarly, antioxidants and the moment of supplementation did not affect (P> 0.05) the total number of blastomeres (86.2-90.5 and 84.4-90.5, respectively) compared with control (85.7). However, the percentage of apoptotic cells was reduced (P< 0.05) in groups supplemented during IVM (1.7%), IVC (2.0%) or both (1.8%) compared with control (4.3%). Intracellular ROS levels measured in Day 7 blastocysts were reduced (P< 0.05) in all groups (0.60-0.78), with the exception of the group supplemented with beta-mercaptoethanol during IVC (0.88), which did not differ (P> 0.05) from that in the control group (1.00). Re-expansion rates were not affected (P> 0.05) by the treatments (50.0%-93.0%). In conclusion, antioxidant supplementation during IVM and/or IVC reduces intracellular ROS and the rate of apoptosis; however, supplementation does not increase embryonic development and survival after vitrification. (AU)

• Processo FAPESP: 11/18257-2 - Criotolerância de embriões bovinos produzidos in vitro: efeitos da tensão de oxigênio e da suplementação do meio com antioxidantes em diferentes etapas do cultivo
• Beneficiário: Gisele Zoccal Mingoti
• Modalidade de apoio: Auxílio à Pesquisa - Regular