Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Reduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in the presence of antioxidants

Full text
Author(s):
Rocha-Frigoni, Nathalia A. S. [1] ; Leao, Beatriz C. S. [1] ; Nogueira, Eriklis [2] ; Accorsi, Monica F. [1] ; Mingoti, Gisele Z. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, UNESP, Dept Anim Hlth, Sch Vet Med, BR-16050680 Aracatuba, SP - Brazil
[2] Brazilian Agr Res Corp, EMBRAPA Pantanal, BR-79320900 Corumba, MS - Brazil
Total Affiliations: 2
Document type: Journal article
Source: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 26, n. 6, p. 797-805, 2014.
Web of Science Citations: 11
Abstract

The effects of intracellular (cysteine and beta-mercaptoethanol) and extracellular (catalase) antioxidant supplementation at different times during in vitro production (IVM and/or in vitro culture (IVC)) on bovine embryo development, intracellular reactive oxygen species (ROS) levels, apoptosis and re-expansion rates after a vitrification-thawing process were examined. Blastocyst frequencies were not affected by either antioxidant supplementation (40.5%56.4%) or the timing of supplementation (41.7%-55.4%) compared with control (48.7%; P>0.05). Similarly, antioxidants and the moment of supplementation did not affect (P> 0.05) the total number of blastomeres (86.2-90.5 and 84.4-90.5, respectively) compared with control (85.7). However, the percentage of apoptotic cells was reduced (P< 0.05) in groups supplemented during IVM (1.7%), IVC (2.0%) or both (1.8%) compared with control (4.3%). Intracellular ROS levels measured in Day 7 blastocysts were reduced (P< 0.05) in all groups (0.60-0.78), with the exception of the group supplemented with beta-mercaptoethanol during IVC (0.88), which did not differ (P> 0.05) from that in the control group (1.00). Re-expansion rates were not affected (P> 0.05) by the treatments (50.0%-93.0%). In conclusion, antioxidant supplementation during IVM and/or IVC reduces intracellular ROS and the rate of apoptosis; however, supplementation does not increase embryonic development and survival after vitrification. (AU)

FAPESP's process: 11/18257-2 - Cryopreservation of bovine embryos cultured under different oxygen tensions with or without addition of antioxidants in differents steps of culture
Grantee:Gisele Zoccal Mingoti
Support Opportunities: Regular Research Grants