Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius

Autor(es)

Massi, Fernanda Pelisson ^[1] ; Cameiro Vieira, Maria Lucia ^[2] ; Sartori, Daniele ^[1] ; Silva Penha, Rafael Elias ^[1] ; Munhoz, Carla de Freitas ^[2] ; Ferreira, Josue Maldonado ^[1] ; Iamanaka, Beatriz Thie ^[3] ; Taniwaki, Marta Hiromi ^[3] ; Frisvad, Jens C. ^[4] ; Pelegrinelli Fungaro, Maria Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Ctr Ciencias Biolog, BR-86051990 Londrina - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Dept Genet, BR-13400970 Piracicaba - Brazil

^[3] Inst Tecnol Alimentos, BR-13070178 Campinas, SP - Brazil

^[4] Tech Univ Denmark, Dept Syst Biol, DK-2800 Lyngby - Denmark

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Food Microbiology; v. 186, p. 14-21, SEP 1 2014.
• Citações Web of Science: 12

Resumo

The exploitation of the Brazil nut is one of the most important activities of the extractive communities of the Amazon rainforest. However, its commercialization can be affected by the presence of aflatoxins produced by fungi, namely Aspergillus section Flavi. In the present study, we investigated a collection of Aspergillus nomius strains isolated from Brazil nuts using different approaches, including morphological characters, RAPD and AFLP profiles, partial beta-tubulin and calmodulin nucleotide sequences, aflatoxin patterns, as well as tolerance to low water activity in cultured media. Results showed that most of the isolates do belong to A. nomius species, but a few were re-identified as Aspergillus pseudonomius, a very recently described species. The results of the analyses of molecular variance, as well as the high pairwise F-ST values between A. nomius and A. pseudonomius suggested the isolation between these two species and the inexistence of gene flow. Fixed interspecific nucleotide polymorphisms at beta-tubulin and calmodulin loci are presented. All A. pseudonomius strains analyzed produced aflatoxins AFB1, AFB2, AFG1 and AFG2. This study contains the first-ever report on the occurrence in Brazil nuts of A. pseudonomius. The G-type aflatoxins and the mycotoxin tenuazonic acid are reported here for the first time in A. pseudonomius. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/13349-1 - Visita do Dr. Jens C. Frisvad da Denmark Technical University ao Brasil
• Beneficiário: Marta Hiromi Taniwaki
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional