A Novel Cell Line Derived from Pleomorphic Adenoma Expresses MMP2, MMP9, TIMP1, TIMP2, and Shows Numeric Chromosomal Anomalies

Autor(es):Mostrar menos -

Carreira Falcao, Aline Semblano ^[1] ; da Silva Kataoka, Maria Sueli ^[1] ; Bailao Ribeiro, Nelson Antonio ^[2] ; Picanco Diniz, Jr., Jose Antonio ^[3] ; Alves, Jr., Sergio Melo ^{[1, 4]} ; Ribeiro Ribeiro, Andre L. ^{[1, 4]} ; de Siqueira, Adriane Sousa ^[5] ; da Silva, Artur Luiz ^[6] ; Juca Ramos, Rommel Thiago ^[6] ; Freitas, Vanessa M. ^[5] ; Jaeger, Ruy G. ^[5] ; Pinheiro, Joao J. V. ^{[5, 1]}

Afiliação do(s) autor(es)

^[1] Fed Univ Para UFPA, Sch Dent, Dept Oral & Maxillofacial Pathol, Belem, Para - Brazil

^[2] Evandro Chagas Inst, Mol Biol Lab, Belem, Para - Brazil

^[3] Evandro Chagas Inst, Electron Microscopy Lab, Belem, Para - Brazil

^[4] Univ Ctr Para CESUPA, Sch Dent, Dept Oral & Maxillofacial Surg, Belem, Para - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil

^[6] Univ Fed Para UFPA, Fed Univ Para, Inst Biol Sci, Belem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 8 AUG 19 2014.
• Citações Web of Science: 6

Resumo

Pleomorphic adenoma is the most common salivary gland neoplasm, and it can be locally invasive, despite its slow growth. This study aimed to establish a novel cell line (AP-1) derived from a human pleomorphic adenoma sample to better understand local invasiveness of this tumor. AP-1 cell line was characterized by cell growth analysis, expression of epithelial and myoepithelial markers by immunofluorescence, electron microscopy, 3D cell culture assays, cytogenetic features and transcriptomic study. Expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs)was also analyzed by immunofluorescence and zymography. Furthermore, epithelial and myoepithelial markers, MMPs and TIMPs were studied in the tumor that originated the cell line. AP-1 cells showed neoplastic epithelial and myoepithelial markers, such as cytokeratins, vimentin, S100 protein and smooth-muscle actin. These molecules were also found in vivo, in the tumor that originated the cell line. MMPs and TIMPs were observed in vivo and in AP-1 cells. Growth curve showed that AP-1 exhibited a doubling time of 3.342 days. AP-1 cells grown inside Matrigel recapitulated tumor architecture. Different numerical and structural chromosomal anomalies were visualized in cytogenetic analysis. Transcriptomic analysis addressed expression of 7 target genes (VIM, TIMP2, MMP2, MMP9, TIMP1, ACTA2 e PLAG1). Results were compared to transcriptomic profile of nonneoplastic salivary gland cells (HSG). Only MMP9 was not expressed in both libraries, and VIM was expressed solely in AP-1 library. The major difference regarding gene expression level between AP-1 and HSG samples occurred for MMP2. This gene was 184 times more expressed in AP-1 cells. Our findings suggest that AP-1 cell line could be a useful model for further studies on pleomorphic adenoma biology. (AU)

• Processo FAPESP: 09/17336-6 - Peptídeos bioativos da laminina induzindo invadopódios em linhagem celular derivada de carcinoma epidermóide: estudo dinâmico por microscopia 4D
• Beneficiário: Adriane Sousa de Siqueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado