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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

CD99 is upregulated in placenta and astrocytomas with a differential subcellular distribution according to the malignancy stage

Texto completo
Autor(es):
Urias, Ursula [1] ; Marie, Suely K. N. [1, 2] ; Uno, Miyuki [1] ; da Silva, Roseli [1] ; Evagelinellis, Maria M. [1] ; Caballero, Otavia L. [3] ; Stevenson, Brian J. [4] ; Silva, Jr., Wilson A. [5, 6] ; Simpson, Andrew J. [7] ; Oba-Shinjo, Sueli M. [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med, Dept Neurol, Lab Mol & Cellular Biol, BR-01246903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Ctr Studies Cellular & Mol Therapy NETCEM, BR-01246903 Sao Paulo - Brazil
[3] Mem Sloan Kettering Canc Ctr, New York Branch, Ludwig Inst Canc Res, New York, NY 10065 - USA
[4] Univ Lausanne, Ludwig Inst Canc Res, CH-1015 Lausanne - Switzerland
[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Ctr Integrat Syst Biol CISBi NAP, BR-14049900 Ribeirao Preto, SP - Brazil
[7] Ludwig Inst Canc Res, New York, NY 10017 - USA
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF NEURO-ONCOLOGY; v. 119, n. 1, p. 59-70, AUG 2014.
Citações Web of Science: 4
Resumo

In the present study, we searched for genes highly expressed in placenta and that could contribute to the establishment and maintenance of a malignant phenotype in different types of tumours, and in astrocytomas in particular. We employed a strategy based on the integration of in silico data from previously generated massively parallel signature sequencing and public serial analysis of gene expression databases. Among 12 selected genes, CD99 exhibited the highest relative mRNA expression in GBM compared to non-neoplastic brain tissues. In a larger cohort of astrocytic tumours, we further demonstrated increased CD99 expression in all malignant grades, with GBMs showing the highest values. These findings were confirmed at the protein level by Western blotting and immunohistochemistry. Additionally, we demonstrated the CD99 localisation profile in astrocytic tumours. Interestingly, CD99 expression was confined to the cytoplasm or membrane in more malignant astrocytomas, in contrast to non-neoplastic brain tissue or non-infiltrative pilocytic astrocytoma, which showed no obvious staining in these structures. Comparison of three GBM cell lines revealed higher CD99 expression at the membrane and higher migratory capacity in the A172 and U87MG lines, but lower CD99 expression and no migratory ability in the T98 line. Knocking down CD99 expression by siRNA decreased significantly the migration of both cell lines. These integrated CD99 gene and protein expression results suggest that CD99 expression in astrocytomas of different malignant grades might contribute to the infiltrative ability and support the importance of CD99 as a potential target to reduce infiltrative astrocytoma capacity in migration and invasion. (AU)

Processo FAPESP: 06/56989-7 - Estudo da expressao aberrante em tumores do sistema nervoso central de genes envolvidos em placenta
Beneficiário:Sueli Mieko Oba Shinjo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 04/12133-6 - Procura de marcadores moleculares relacionados ao diagnóstico e prognóstico de tumores do sistema nervoso central
Beneficiário:Suely Kazue Nagahashi Marie
Linha de fomento: Auxílio à Pesquisa - Temático