Amelioration of the autonomic imbalances of old age with exercise: exploring the molecular and physiological mechanisms

• Processo: 11/51410-9
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Data de Início da vigência: 01 de maio de 2012
• Data de Término da vigência: 31 de julho de 2016
• Área do conhecimento: Ciências Biológicas - Fisiologia - Fisiologia de Órgãos e Sistemas
• Acordo de Cooperação: BBSRC, UKRI
• Pesquisador responsável: Lisete Compagno Michelini
• Beneficiário: Lisete Compagno Michelini
• Pesquisador Responsável no exterior: David Murphy
• Instituição Parceira no exterior: University of Bristol, Inglaterra
• Instituição Sede: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Bolsa(s) vinculada(s): 15/07863-0 - Melhora das alterações autonômicas com o exercício físico durante o processo de envelhecimento: exploração dos mecanismos moleculares e fisiológicos, BP.PD; 14/19515-3 - Melhoria dos desequilíbrios autonômicos da velhice com exercício explorando os mecanismos moleculares e fisiológicos, BP.TT; 13/02016-1 - Treinamento técnico para capacitação de alunos em técnicas de pesquisa em fisiologia do esforço e cardiovascular, BP.TT; 12/10326-8 - Evolução temporal do controle autonômico e respostas cardiovasculares associadas em SHR jovens submetidos ao treinamento aeróbio, BP.DR
• Assunto(s): Fisiologia cardiovascular Sedentarismo Velhice Idosos Atividade física
• Publicação FAPESP: https://media.fapesp.br/bv/uploads/pdfs/International...RCUK_2_8_9.pdf

Resumo

There is considerable anecdotal evidence that a sedentary Iifestyle can lead to ill heath in old age, whereas physical activity has long-term health and wellbeing benefits. The reasons why homeostatic systems deteriorate in the elderly, and why exercise can ameliorate this deterioration, are not well understood. However, there is growing evidence that autonomic nervous system (ANS) imbalance is involved in a wide range of chronic conditions of old-age, such as hypertension. We hypothesize that the development and/or maintenance of the ANS imbalance is associated with changes in the expression of genes in the brain that, through their activity, form one or more functional networks can be influenced by both age and lifestyle factors, such as physical activity. In order to test this hypothesis, we will: i) identify putative gene networks by carrying out detailed and comprehensive transcriptome analysis on brain regions involved in the regulation of ANS of WKY rats compared to SHRs with a genetic predisposition to ANS imbalance characterized by over sympathetic activity related to gene expression changes as animals' age; iii) ask if gene expression is altered by physical exercise, initiated either before or after the onset of ANS imbalance; Iv) use bioinformatic tools to construct putative pathways and gene networks that may govern these processes to identify hub genes with many connections. Proof of principle studies will determine whether some of the age-related pathophysiology can be ameliorated. If so, these genes will undergo robust molecular, cellular and, physiological analysis that will reveal the roles of these hub genes in network stability and their response to external cues such as exercise. (AU)

Publicações científicas

(12)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

ICHIGE, MARCELO H. A.; SANTOS, CARLA R.; JORDAO, CAMILA P.; CERONI, ALEXANDRE; NEGRAO, CARLOS E.; MICHELINI, LISETE C.. Exercise training preserves vagal preganglionic neurones and restores parasympathetic tonus in heart failure. JOURNAL OF PHYSIOLOGY-LONDON, v. 594, n. 21, p. 6241-6254, NOV 2016. (14/06772-8, 11/51410-9)

DAMPNEY, ROGER A.; MICHELINI, LISETE C.; LI, DE-PEI; PAN, HUI-LIN. Regulation of sympathetic vasomotor activity by the hypothalamic paraventricular nucleus in normotensive and hypertensive states. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, v. 315, n. 5, p. H1200-H1214, NOV 2018. (15/24935-4, 11/51410-9)

RAQUEL, HIVINY DE A.; MASSON, GUSTAVO S.; BARNA, BARBARA FALQUETTO; ZANLUQUI, NAGELA G.; PINGE-FILHO, PHILENO; MICHELINI, LISETE C.; MARTINS-PINGE, MARLI C.. Swimming Training Modulates Nitric Oxide-Glutamate Interaction in the Rostral Ventrolateral Medulla in Normotensive Conscious Rats. FRONTIERS IN PHYSIOLOGY, v. 7, JUN 13 2016. (11/51410-9)

CHAAR, LAIALI JURDI; ALVES, TATIANA PEREIRA; BATISTA JUNIOR, ALVARO MARTINS; MICHELINI, LISETE COMPAGNO. Early Training-Induced Reduction of Angiotensinogen in Autonomic Areas-The Main Effect of Exercise on Brain Renin-Angiotensin System in Hypertensive Rats. PLoS One, v. 10, n. 9, SEP 15 2015. (09/54299-1, 09/03708-9, 11/51410-9)

SILVA, JR., SEBASTIAO D.; JARA, ZAIRA P.; PERES, ROSELI; LIMA, LARISSA S.; SCAVONE, CRISTOFORO; MONTEZANO, AUGUSTO C.; TOUYZ, RHIAN M.; CASARINI, DULCE E.; MICHELINI, LISETE C.. Temporal changes in cardiac oxidative stress, inflammation and remodeling induced by exercise in hypertension: Role for local angiotensin II reduction. PLoS One, v. 12, n. 12, DEC 12 2017. (11/15681-8, 10/51904-9, 11/51410-9)

SANTOS, C. R.; RUGGERI, A.; CERONI, A.; MICHELINI, L. C.. Exercise training abrogates age-dependent loss of hypothalamic oxytocinergic circuitry and maintains high parasympathetic activity. Journal of Neuroendocrinology, v. 30, n. 8, SI, AUG 2018. (15/24935-4, 11/51410-9, 11/15676-4)

DE MOLO, VITOR U.; SALDANHA, RAYSSA R. M.; DOS SANTOS, CARLA R.; CRUZ, JOSIANE DE CAMPOS; LIRA, VITOR A.; SANTANA-FILHO, VALTER J.; MICHELINI, LISETE C.. Ovarian Hormone Deprivation Reduces Oxytocin Expression in Paraventricular Nucleus Preautonomic Neurons and Correlates with Baroreflex Impairment in Rats. FRONTIERS IN PHYSIOLOGY, v. 7, OCT 13 2016. (11/51410-9)

MICHELINI, LISETE C.; O'LEARY, DONAL S.; RAVEN, PETER B.; NOBREGA, ANTONIO C. L.. Neural control of circulation and exercise: a translational approach disclosing interactions between central command, arterial baroreflex, and muscle metaboreflex. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, v. 309, n. 3, p. H381-H392, AUG 1 2015. (11/51410-9)

BUTTLER, LEILA; JORDAO, MARIA T.; FRAGAS, MATHEUS G.; RUGGERI, ADRIANA; CERONI, ALEXANDRE; MICHELINI, LISETE C.. Maintenance of Blood-Brain Barrier Integrity in Hypertension: A Novel Benefit of Exercise Training for Autonomic Control. FRONTIERS IN PHYSIOLOGY, v. 8, DEC 12 2017. (11/14395-1, 15/24935-4, 11/51410-9)

SANTOS, C. R.; RUGGERI, A.; CERONI, A.; MICHELINI, L. C.. Exercise training abrogates age-dependent loss of hypothalamic oxytocinergic circuitry and maintains high parasympathetic activity. Journal of Neuroendocrinology, v. 30, n. 8, p. 13-pg., 2018-08-01. (15/24935-4, 11/51410-9, 11/15676-4)

CAMPOS, LUCIANA A.; CIPOLLA-NETO, JOSE; AMARAL, FERNANDA G.; MICHELINI, LISETE C.; BADER, MICHAEL; BALTATU, OVIDIU C.. The Angiotensin-Melatonin Axis. INTERNATIONAL JOURNAL OF HYPERTENSION, v. 2013, p. 7-pg., 2013-01-01. (11/51410-9, 11/50078-0)

DELLACQUA, LAIS OLIVEIRA; MICHELINI, LISETE COMPAGNO; HINDMARCH, CHARLIE; RICHMOND PATON, JULIAN FRANCIS; MURPHY, DAVID; ANTUNES, VAGNER. Improvement of Vagal Tone and Reduction in the Blood Pressure by Physical Training in Aging Spontaneously Hypertensive Rats. FASEB JOURNAL, v. 30, p. 1-pg., 2016-04-01. (11/51410-9, 12/22368-7)