Orthotopic tumorgrafts in nude mice as a model to evaluate calcitriol effects in breast cancer

• Processo: 17/02390-1
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Data de Início da vigência: 01 de maio de 2017
• Data de Término da vigência: 31 de outubro de 2017
• Área do conhecimento: Ciências da Saúde - Medicina - Clínica Médica
• Pesquisador responsável: Maria Aparecida Azevedo Koike Folgueira
• Beneficiário: Maria Aparecida Azevedo Koike Folgueira
• Instituição Sede: Instituto do Câncer do Estado de São Paulo Octavio Frias de Oliveira (ICESP). Coordenadoria de Serviços de Saúde (CSS). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brasil
• Assunto(s): Calcitriol Neoplasias mamárias Oncologia
• Palavra(s)-Chave do Pesquisador: Breast Cancer | calcitriol | proliferation | xenograft | Cancerologia

Resumo

Calcitriol antiproliferative effects were observed in xenografts of breast cancer cell lines, however they were not yet investigated in tumorgrafts, consisting of freshly collected breast cancer samples xenografted into animals.Objectives: To establish a tumorgraft model, from freshly collected breast cancer samples, which were directly implanted in nude mice, to study calcitriol effects.Methods: Breast cancer samples collected from 12 patients were orthotopically implanted into nude mice. Animals were treated with weekly intratumoral injections of calcitriol 3 ¼g/Kg, which was previously shown to induce peak serum calcitriol levels in the predicted therapeutic range.Results: Success engraftment rate was 25%. Tumorgrafts were established from aggressive (HER2 positive or histological grade 3) highly proliferative samples and original tumor characteristics were preserved. Calcitriol highly induced its target gene, CYP24A1, indicating that the genomic vitamin D pathway is active in tumorgrafts. However, no differences in the expression of proliferation and apoptosis markers (BrdU incorporation, Ki67, CDKN1A, CDKN1B, BCL2 expression) were observed in these highly proliferative tumor samples.Conclusions: Tumorgrafts seem a promising model to explore other calcitriol doses and regimens, considering the heterogeneity of the disease and microenvironment interactions. (AU)