Functions of DUSP 12 and ITS relation to FGF2 signaling

• Processo: 06/56896-9
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Data de Início da vigência: 12 de março de 2007
• Data de Término da vigência: 11 de agosto de 2007
• Área de conhecimento: Ciências Biológicas - Bioquímica - Biologia Molecular
• Pesquisador responsável: Fábio Luis Forti
• Beneficiário: Fábio Luis Forti
• Pesquisador Anfitrião: Tomas Mikael Mustelin
• Instituição Sede: Pessoa Física
• Instituição Anfitriã: University of California, San Diego (UC San Diego), Estados Unidos
• Vinculado ao auxílio: 03/02717-8 - Desconstrução molecular do módulo de controle do ciclo celular em células adrenocorticais y1 de camundongo, AP.TEM
• Palavra(s)-Chave do Pesquisador: Dusp | Dusp12 | Fgf | Fgf2 | Signal Transduction

Resumo

This project aims to study the molecular involvement of dusp12 gene in mouse embryonic fibroblasts (MEp) isolated and established from dusp12 gene targeted mouse. The absence of dusp12 gene in cell cycle control proliferation and migration of MEF lines will be investigated. DUSP12 protein expression will be checked as well as phosphorylation (activation) of MAPKs in parental MEFs ou isolated MEF lines over expressing dusp12. Responsiveness; of these MEF lines to FGF2 stimulation will also be investigated as to proliferative and/or antiproliferative FGF2 effects. In order to identify specific substrates or partners of DUSP12 protein to perform its cellular actions, a yeast-two-hybrid screen will be made and identified genes will be over expressed or knockdown by siRNA in MEF and other mouse lines. (AU)