Tyrosyl phosphorylation and vascular permeability during cardiac surgery

• Processo: 99/07234-8
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Data de Início da vigência: 01 de abril de 2000
• Data de Término da vigência: 30 de junho de 2001
• Área de conhecimento: Ciências Biológicas - Farmacologia - Farmacologia Geral
• Pesquisador responsável: Renato Do Rego de Araujo Faro
• Beneficiário: Renato Do Rego de Araujo Faro
• Pesquisador Anfitrião: Frank William Sellke
• Instituição Sede: Pessoa Física
• Instituição Anfitriã: Harvard University, Boston, Estados Unidos
• Palavra(s)-Chave do Pesquisador: Cardioplegia | Microcirculation | Molecular Biology | Permeability

Resumo

The objective of the research is to determine the roles of tyrosyl kinase/phosphatase activities and protein tyrosyl phosphorylation in mediating acute changes in coronary and systemic microvascular permeability, during ischemic cardioplegia and cardiopulmonary bypass (CPB). This work will be accomplished through a reductive approach using molecular and cellular biology techniques to examine the protein tyrosyl phosphorylation and gene expressions involved in maintaining vascular adhesion and integrity in a porcine model of cardioplegia and CPB and in isolated rat microvessels. We will correlate these molecular and cellular changes with in vivo and in vitro indices of vascular permeability. (AU)