Micromorphology of the Dental Pulp Is Highly Preserved in Cancer Patients Who Underwent Head and Neck Radiotherapy

Autor(es):Mostrar menos -

Faria, Karma Morais ^{[1, 2]} ; Brandao, Thais Bianca ^[3] ; Prado Ribeiro, Ana Carolina ^[3] ; Gouvea Vasconcellos, Adriele Ferreira ^[3] ; de Carvalho, Icaro Thiago ^[4] ; de Arruda, Fernando Freire ^[4] ; Castro Junior, Gilberto ^[5] ; Gross, Vanessa Cristina ^{[1, 2]} ; Almeida, Oslei Paes ^{[1, 2]} ; Lopes, Marcio Ajudarte ^{[1, 2]} ; Santos-Silva, Alan Roger ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Dept Oral Diag, Semiol Area, Piracicaba Dent Sch, BR-13414903 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Dept Oral Diag, Pathol Area, Piracicaba Dent Sch, BR-13414903 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Inst Canc Estado Sao Paulo, Dental Oncol Serv, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med, Inst Canc Estado Sao Paulo, Radiotherapy Serv, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med, Inst Canc Estado Sao Paulo, Clin Oncol Serv, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 40, n. 10, p. 1553-1559, OCT 2014.
• Citações Web of Science: 11

Resumo

Introduction: Teeth are often included in the radiation field during head and neck radiotherapy, and recent clinical evidence suggests that dental pulp is negatively affected by the direct effects of radiation, leading to impaired sensitivity of the dental pulp: Therefore, this study aimed to investigate the direct effects of radiation on the microvasculature, innervation, and extracellular matrix of the dental pulp of patients who have undergone head and neck radiotherapy. Methods: Twenty-three samples of dental pulp from patients who finished head and neck radiotherapy were analyzed. Samples were histologically processed and stained with hematoxylin-eosin for morphologic evaluation of the microvasculature, innervation, and extracellular matrix. Subsequently, immunohistochemical analysis of proteins related to vascularization (CD34 and smooth muscle actin), innervation (S-100, NCAM/CD56, and neurofilament), and extracellular matrix (vimentin) of the dental pulp was performed. Results: The morphologic study identified preservation of the microvasculature, nerve bundles, and components of the extracellular matrix in all studied samples. The immunohistochemical analysis confirmed the morphologic findings and showed a normal pattern of expression for the studied proteins in all samples. Conclusions: Direct effects of radiotherapy are not able to generate morphologic changes in the microvasculature, innervation, and extracellular matrix components of the dental pulp in head and neck cancer patients. (AU)

• Processo FAPESP: 12/06138-1 - Estudo prospectivo da incidência de trismo induzido por radioterapia em pacientes com carcinoma espinocelular de boca
• Beneficiário: Ana Carolina Prado Ribeiro e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/18402-8 - Avaliação dos efeitos diretos da radiação sobre o esmalte, a dentina e a polpa dental de pacientes com câncer de cabeça e pescoço
• Beneficiário: Alan Roger dos Santos Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular