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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Structure of a bacterial alpha(2)-macroglobulin reveals mimicry of eukaryotic innate immunity

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Autor(es):
Wong, Steve G. [1, 2, 3] ; Dessen, Andrea [1, 2, 3, 4]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble - France
[2] CNRS, IBS, F-38044 Grenoble - France
[3] CEA, IBS, F-38044 Grenoble - France
[4] Brazilian Natl Lab Biosci LNBio, CNPEM, BR-13083100 Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: NATURE COMMUNICATIONS; v. 5, SEP 2014.
Citações Web of Science: 17
Resumo

Alpha-2-macroglobulins (A2Ms) are plasma proteins that trap and inhibit a broad range of proteases and are major components of the eukaryotic innate immune system. Surprisingly, A2M-like proteins were identified in pathogenically invasive bacteria and species that colonize higher eukaryotes. Bacterial A2Ms are located in the periplasm where they are believed to provide protection to the cell by trapping external proteases through a covalent interaction with an activated thioester. Here we report the crystal structures and characterization of Salmonella enterica ser. Typhimurium A2M in different states of thioester activation. The structures reveal thirteen domains whose arrangement displays high similarity to proteins involved in eukaryotic immune defence. A structural lock mechanism maintains the stability of the buried thioester, a requirement for its protease-trapping activity. These findings indicate that bacteria have developed a rudimentary innate immune system whose mechanism mimics that of eukaryotes. (AU)

Processo FAPESP: 11/52067-6 - Estruturação de complexos macromoleculares da parede bacteriana: biossíntese e virulência
Beneficiário:Andrea Dessen de Souza e Silva
Linha de fomento: Auxílio à Pesquisa - Programa SPEC