The Flavonols Quercetin, Myricetin, Kaempferol, and Galangin Inhibit the Net Oxygen Consumption by Immune Complex-Stimulated Human and Rabbit Neutrophils

Autor(es)

Figueiredo-Rinhel, Andrea S. G. ^[1] ; Santos, Everton O. L. ^[1] ; Kabeya, Luciana M. ^[1] ; Azzolini, Ana Elisa C. S. ^[1] ; Simoes-Ambrosio, Livia M. C. ^[1] ; Lucisano-Valim, Yara M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Quim & Fis, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES; v. 69, n. 7-8, p. 346-356, JUL-AUG 2014.
• Citações Web of Science: 7

Resumo

Stimulated human neutrophils exhibit increased net oxygen consumption (NOC) due to the conversion of O-2 into the superoxide anion by the NADPH oxidase enzymatic complex during the respiratory burst. In several inflammatory diseases, overproduction of these oxidants causes tissue damage. The present study aims to: (a) optimize the experimental conditions used to measure the NOC in serum-opsonized zymosan (OZ)- and insoluble immune complex (i-IC)-stimulated human and rabbit neutrophils; and (b) compare the effect of four flavonols (quercetin, myricetin, kaempferol, and galangin) on this activity. We used a Clark-type oxygen electrode to measure the NOC of stimulated neutrophils. Eliciting the neutrophil respiratory burst with OZ and i-IC yielded similar maximum O-2 uptake levels within the same species, but the human neutrophil NOC was almost four times higher than the rabbit neutrophil NOC. The optimal experimental conditions established for both cell types were 4 . 10(6) neutrophils mL(-1), 2 mg mL(-1) OZ, and 240 mu g mL(-1) i-IC. Upon stimulation with OZ or i-IC, the tested flavonols reduced the human and rabbit neutrophil NOC in the same order of potency quercetin and galangin were the most and the least potent, respectively. These compounds were around four times more effective in inhibiting the rabbit as compared to the human neutrophil NOC, respectively. The four flavonols were not toxic to human or rabbit neutrophils. The experimental conditions used are suitable for both the determination of human and rabbit neutrophil NOC and for the assessment of the modulatory effects of natural compounds on these activities. The relationship between the level of NOC and the inhibitory potency of the flavonols suggests that rabbit neutrophils can be useful experimental models to predict the effect of drugs on immune complex-stimulated human neutrophils. (AU)

• Processo FAPESP: 04/01962-1 - Construção de um anticorpo quimérico bi-especifico, anti-fcgamariii e anti-BSA, para avaliar a resposta de neutrófilos, de indivíduos do alótipo FcgamaRIIa-R131, a estimulação com imunocomplexos contendo IgG2
• Beneficiário: Yara Maria Lucisano Valim
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/00840-8 - Estudo do mecanismo de ação de derivados cumarínicos sobre a atividade da mieloperoxidase: investigação da metabolização dessas substâncias a radicais livres fenoxílicos, implicações no estresse oxidativo e na modulação de funções de leucócitos
• Beneficiário: Luciana Mariko Kabeya
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado