Modification of the brain proteome of Africanized honeybees (Apis mellifera) exposed to a subaEurolethal doses of the insecticide fipronil

Autor(es)

Roat, T. C. ^[1] ; dos Santos-Pinto, J. R. A. ^[1] ; dos Santos, L. D. ^[2] ; Santos, K. S. ^[3] ; Malaspina, O. ^[1] ; Palma, M. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State UNESP, Ctr Study Social Insects, Dept Biol, Inst Biosci Rio Claro, Rio Claro, SP - Brazil

^[2] Univ Sao Paulo State UNESP, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP - Brazil

^[3] Discipline Allergy & Immunol HC Incor FMUSP, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY; v. 23, n. 9, p. 1659-1670, NOV 2014.
• Citações Web of Science: 11

Resumo

Fipronil is a phenylpyrazole insecticide that is widely used in Brazilian agriculture for pest control. Although honeybees are not targets of fipronil, studies indicate that this pesticide can be harmful to honeybees. To assess the effects of fipronil in the brain of Africanized Apis mellifera workers, this study focused on the toxico-proteome profiling of the brain of newly emerged and aged honeybee workers that were exposed to a sub-lethal dose (10 pg fipronil per day. i.e. (1)/(100) of LD50/bee/day during 5 days) of the insecticide. Proteomic analysis identified 25 proteins that were differentially up-regulated or down-regulated when the fipronil-exposed and non-exposed groups were compared. These proteins are potentially related to pathogen susceptibility, neuronal chemical stress, neuronal protein misfolding, and occurrence of apoptosis, ischemia, visual impairment, damaged synapse formation, brain degeneration, memory and learning impairment. The exposure of honeybees to a very low dose of fipronil, even for a short period of time (5 days), was sufficient to cause a series of important neuroproteomic changes in the brains of honeybees. (AU)

• Processo FAPESP: 11/51684-1 - Biologia de sistemas como estratégia experimental para a descoberta de novos produtos naturais na fauna de artrópodes peçonhentos do Estado de São Paulo
• Beneficiário: Mario Sergio Palma
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 12/13370-8 - Como o cérebro de Apis mellifera (Lineu, 1758) (Hymenoptera, Apidae) responde à dose subletal de tiametoxam?
• Beneficiário: Thaisa Cristina Roat
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 08/05018-7 - Efeitos toxicológicos do inseticida fipronil em operárias e rainhas de Apis mellifera (Hymenoptera: Apidae): atividade neural e proteínas de desintoxicação
• Beneficiário: Thaisa Cristina Roat
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado