Influence of N-Acetylcysteine on Oxidative Stress in Slow-Twitch Soleus Muscle of Heart Failure Rats

Autor(es):Mostrar menos -

Martinez, Paula F. ^{[1, 2]} ; Bonomo, Camila ^[1] ; Guizoni, Daniele M. ^[1] ; Oliveira Junior, Silvio A. ^{[1, 2]} ; Damatto, Ricardo L. ^[1] ; Cezar, Marcelo D. M. ^[1] ; Lima, Aline R. R. ^[1] ; Pagan, Luana U. ^[1] ; Seiva, Fabio R. ^[3] ; Fernandes, Denise C. ^[4] ; Laurindo, Francisco R. M. ^[4] ; Novelli, Ethel L. B. ^[1] ; Matsubara, Luiz S. ^[1] ; Zornoff, Leonardo A. M. ^[1] ; Okoshi, Katashi ^[1] ; Okoshi, Marina P. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Fed Mato Grosso do Sul, Campo Grande - Brazil

^[3] North Parana State Univ, Bandeirantes - Brazil

^[4] Heart Inst InCor HC FMUSP, Vasc Biol Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY; v. 35, n. 1, p. 148-159, 2015.
• Citações Web of Science: 19

Resumo

Background: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI, rats were assigned to Sham, MI-C (without treatment), and MI-NAC (treated with N-acetylcysteine) groups. Two months later, echocardiogram showed left ventricular dysfunction in MI-C; NAC attenuated diastolic dysfunction. In soleus muscle, glutathione peroxidase and superoxide dismutase activity was decreased in MI-C and unchanged by NAC. 3-nitrotyrosine was similar in MI-C and Sham, and lower in MI-NAC than MI-C. Total reactive oxygen species (ROS) production was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products. The 2-hydroxyethidium (EOH)/DHE ratio did not differ between Sham and MI-C and was higher in MI-NAC. The ethidium/DHE ratio was higher in MI-C than Sham and unchanged by NAC. NADPH oxidase activity was similar in Sham and MI-C and lower in MI-NAC. Gene expression of p47(phox) was lower in MI-C than Sham. NAC decreased NOX4 and p22(phox) expression. Conclusions: We corroborate the case that oxidative stress is increased in skeletal muscle of heart failure rats and show for the first time that oxidative stress is not related to increased NADPH oxidase activity. Copyright (C) 2015 S. Karger AG, Basel (AU)

• Processo FAPESP: 07/59500-1 - Participacao do estresse oxidativo na modulacao dos fatores de regulacao miogenica na musculatura esqueletica de ratos com insuficiencia cardiaca cronica.
• Beneficiário: Paula Felippe Martinez
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/50461-6 - Participacao do estresse oxidativo na modulacao dos fatores de regulacao miogenica na musculatura esqueletica de ratos com insuficiencia cardiaca cronica.
• Beneficiário: Marina Politi Okoshi
• Modalidade de apoio: Auxílio à Pesquisa - Regular