Transcorneal iontophoresis of dendrimers: PAMAM corneal penetration and dexamethasone delivery

Autor(es)

Souza, Joel G. ^[1] ; Dias, Karina ^[1] ; Silva, Silas A. M. ^[2] ; de Rezende, Lucas C. D. ^[1] ; Rocha, Eduardo M. ^[3] ; Emery, Flavio S. ^[1] ; Lopez, Renata F. V. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Med, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Ophthalmol, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CONTROLLED RELEASE; v. 200, p. 115-124, FEB 28 2015.
• Citações Web of Science: 24

Resumo

Iontophoresis of nanocarriers in the eye has been proposed to sustain drug delivery and maintain therapeutic concentrations. Fourth generation polyamidoamine (PAMAM) dendrimers are semi-rigid nanoparticles with surface groups that are easily modified. These dendrimers are known to modulate tight junctions, increase paracellular transport of small molecules and be translocated across epithelial barriers, exhibiting high uptake by different cell lines. The first aim of this study was to investigate the effect of iontophoresis on PAMAM penetration and distribution into the cornea. The second aim was to evaluate, ex vivo and in vivo, the effect of these dendrimers in dexamethasone (Dex) transcorneal iontophoresis. Anionic (PAMAM G3.5) and cationic (PAMAM G4) dendrimers were labeled with fluorescein isothiocyanate (FITC), and their distribution in the cornea was investigated using confocal microscopy after ex vivo anodal and cathodal iontophoresis for various application times. The particle size distribution and zeta potential of the dendrimers in an isosmotic solution were determined using dynamic light scattering and Nanoparticle Tracking Analysis (NTA), where the movement of small particles and the formation of large aggregates, from 5 to 100 nm, could be observed. Transcorneal iontophoresis increased the intensity and depth of PAMAM-FITC fluorescence in the cornea, suggesting improved transport of the dendrimers across the epithelium toward the stroma. PAMAM complexes with Dex were characterized by C-13-NMR, H-1-NMR and DOSY. PAMAMG3.5 and PAMAMG4 increased the aqueous solubility of Dex by 10.3 and 3.9-fold, respectively; however, the particle size distribution and zeta potential remained unchanged. PAMAM G3.5 decreased the Dex diffusion coefficient 48-fold compared with PAMAM G4. The ex vivo studies showed that iontophoresis increased the amount of Dex that penetrated into the cornea by 2.9, 5.6 and 3.0-fold for Dex, Dex-PAMAM G4 and Dex-PAMAM G3.5, respectively. In vivo experiments, however, revealed that iontophoresis of Dex-PAMAM-G3.5 increased Dex concentration in the aqueous humor by 6.6-fold, while iontophoresis of Dex-PAMAM G4 and Dex increased it 2.5 and 2-fold, respectively. Therefore, iontophoresis targeted PAMAM to the cornea but it is the sustained delivery of the Dex from PAMAM that prevents its rapid elimination from the aqueous humor. In conclusion, iontophoresis of PAMAM complexes represents a promising strategy for targeted and sustained topical drug delivery to the eye. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/19210-7 - Avaliação do potencial da associação de dendrímeros e iontoforese para a administração ocular de fármacos
• Beneficiário: Joel Gonçalves de Souza
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/11305-1 - Dendrímeros como nanocarreadores na administração ocular de fármacos usando iontoforese
• Beneficiário: Renata Fonseca Vianna Lopez
• Linha de fomento: Auxílio à Pesquisa - Regular