A metabolomic protocol for plant systematics by matrix-assisted laser-desorption/ionization time-of flight mass spectrometry

Autor(es)

Ernst, Madeleine ^[1] ; Silva, Denise B. ^[1] ; Silva, Ricardo ^[2] ; Monge, Marcelo ^[3] ; Semir, Joao ^[3] ; Vencio, Ricardo Z. N. ^[2] ; Lopes, Norberto P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Chem & Phys, Nucleo Pesquisa Prod Nat & Sintet, BR-14040903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Comp & Math, FFCLRP, BR-14040903 Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytica Chimica Acta; v. 859, p. 46-58, FEB 15 2015.
• Citações Web of Science: 3

Resumo

Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) has been widely used for the identification and classification of microorganisms based on their proteomic fingerprints. However, the use of MALDI-TOF MS in plant research has been very limited. In the present study, a first protocol is proposed for metabolic fingerprinting by MALDI-TOF MS using three different MALDI matrices with subsequent multivariate data analysis by in-house algorithms implemented in the R environment for the taxonomic classification of plants from different genera, families and orders. By merging the data acquired with different matrices, different ionization modes and using careful algorithms and parameter selection, we demonstrate that a close taxonomic classification can be achieved based on plant metabolic fingerprints, with 92% similarity to the taxonomic classifications found in literature. The present work therefore highlights the great potential of applying MALDI-TOF MS for the taxonomic classification of plants and, furthermore, provides a preliminary foundation for future research. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/05215-0 - Metabolômica como ferramenta em sistemática: o modelo em arnica
• Beneficiário: Madeleine Ernst
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/14926-4 - Bioinformática aplicada à Bioenergia: anotação probabilística do metaboloma da cana-de-açúcar
• Beneficiário: Ricardo Roberto da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/18031-7 - Aplicação de MALDI-MS/MS na análise da vicenina-2 e correlatos: da compreensão dos processos ionizantes à geração de imagens
• Beneficiário: Denise Brentan da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/54098-6 - EMU: aquisição de um espectrômetro de massas para geração de imagens na Central de Espectrometria de Massas (mass-facility) da FCFRP-USP: estudos de localização molecular de substâncias biologicamente ativas
• Beneficiário: Norberto Peporine Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários