| Texto completo | |
| Autor(es): |
Chuffa, Luiz Gustavo A.
[1]
;
Fioruci-Fontanelli, Beatriz A.
[1]
;
Mendes, Leonardo O.
[1]
;
Ferreira Seiva, Fabio R.
[2]
;
Martinez, Marcelo
[3]
;
Favaro, Wagner J.
[4]
;
Domeniconi, Raquel F.
[1]
;
Pinheiro, Patricia F. F.
[1]
;
dos Santos, Lucilene Delazari
[5]
;
Martinez, Francisco Eduardo
[3]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista, UNESP, Inst Biosci, Dept Anat, Botucatu, SP - Brazil
[2] State Univ North Parana, Inst Biol, UENP, Bandeirantes, PR - Brazil
[3] Univ Fed Sao Carlos, UFSCar, Dept Morphol & Pathol, BR-13560 Sao Carlos, SP - Brazil
[4] Univ Estadual Campinas, Dept Anat Cell Biol & Physiol & Biophys, UNICAMP, Campinas, SP - Brazil
[5] Univ Estadual Paulista, UNESP, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC CANCER; v. 15, FEB 6 2015. |
| Citações Web of Science: | 37 |
| Resumo | |
Background: Toll-like receptors (TLRs) are effector molecules expressed on the surface of ovarian cancer (OC) cells, but the functions of the TLR2/TLR4 signaling pathways in these cells remain unclear. Melatonin (mel) acts as an anti-inflammatory factor and has been reported to modulate TLRs in some aggressive tumor cell types. Therefore, we investigated OC and the effect of long-term mel therapy on the signaling pathways mediated by TLR2 and TLR4 via myeloid differentiation factor 88 (MyD88) and toll-like receptor-associated activator of interferon (TRIF) in an ethanol-preferring rat model. Methods: To induce OC, the left ovary of animals either consuming 10% (v/v) ethanol or not was injected directly under the bursa with a single dose of 100 mu g of 7,12-dimethylbenz(a) anthracene (DMBA) dissolved in 10 mu L of sesame oil. The right ovaries were used as sham-surgery controls. After developing OC, half of the animals received i.p. injections of mel (200 mu g/100 g b.w./day) for 60 days. Results: Although mel therapy was unable to reduce TLR2 levels, it was able to suppress the OC-associated increase in the levels of the following proteins: TLR4, MyD88, nuclear factor kappa B (NFkB p65), inhibitor of NFkB alpha (IkB alpha), IkB kinase alpha (IKK-alpha), TNF receptor-associated factor 6 (TRAF6), TRIF, interferon regulatory factor 3 (IRF3), interferon beta (IFN-beta), tumor necrosis factor alpha (TNF-alpha), and interleukin (IL)-6. In addition, mel significantly attenuated the expression of IkB alpha, NFkB p65, TRIF and IRF-3, which are involved in TLR4-mediated signaling in OC during ethanol intake. Conclusion: Collectively, our results suggest that mel attenuates the TLR4-induced MyD88- and TRIF-dependent signaling pathways in ethanol-preferring rats with OC. (AU) | |
| Processo FAPESP: | 11/19294-9 - Indução tumoral ovariana e influência da melatonina sobre o processo inflamatório via sinalização mediada pelo receptor Toll-like 4 em ratas UChB |
| Beneficiário: | Luiz Gustavo de Almeida Chuffa |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/02466-7 - Indução tumoral ovariana e influência da melatonina sobre o processo inflamatório via sinalização mediada pelo receptor Toll-like 4 em ratas UChB |
| Beneficiário: | Luiz Gustavo de Almeida Chuffa |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |