Electroporation Transiently Decreases GJB2 (Connexin 26) Expression in B16/BL6 Melanoma Cell Line

Autor(es)

Mor Rangel, Marcelo Monte ^[1] ; Chaible, Lucas Martins ^[1] ; Nagamine, Marcia Kazumi ^[1] ; Mennecier, Gregory ^[1] ; Cogliati, Bruno ^[1] ; de Oliveira, Krishna Duro ^[1] ; Fukumasu, Heidge ^{[1, 2]} ; Sinhorini, Idercio Luiz ^[1] ; Mir, Lluis Maria ^[3] ; Zaidan Dagli, Maria Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Lab Expt & Comparat Oncol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Basic Sci, BR-05508900 Sao Paulo - Brazil

^[3] Inst Gustave Roussy, Villejuif - France

• Tipo de documento: Artigo Científico
• Fonte: Journal of Membrane Biology; v. 248, n. 1, p. 47-52, FEB 2015.
• Citações Web of Science: 2

Resumo

Connexins are proteins that form gap junctions. Perturbations in the cell membrane reportedly promote changes in the expression profile of connexins. Electroporation promotes destabilization by applying electrical pulses, and this procedure is used in electrochemotherapy and gene therapy, among others. This in vitro work aimed to study the interference of electroporation on the expression profile of GJB2 (Cx26 gene) and Connexin 26 in melanoma cell line B16/BL6. The techniques of immunocytochemistry, Western blot, and real-time PCR were used. After electroporation, cells showed a transient decrease in GJB2 mRNA. The immunostaining of Cx26 showed no noticeable change after electroporation at different time points. However, Western blot showed a significant reduction in Cx26 30 min after electroporation. Our results showed that electroporation interferes transiently in the expression of Connexin 26 in melanoma and are consistent with the idea that electroporation is a process of intense stress that promotes cell homeostatic imbalance and results in disruption of cell physiological processes such as transcription and translation. (AU)

• Processo FAPESP: 07/59391-8 - Avaliacao da interferencia do fenomeno de eletroporacao na funcao das juncoes gap e na expressao da conexina 26 em cultura de celulas de melanoma b16/bl6.
• Beneficiário: Maria Lucia Zaidan Dagli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/55534-1 - Estudo comparativo da interferência do fenômeno de eletroporação na função das junções Gap e na expressão da conexina 43 nas linhagens de células neoplásicas E9 e não neoplásicas e 10
• Beneficiário: Marcelo Monte Mór Rangel
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto