Involvement of Major Components from Sporothrix schenckii Cell Wall in the Caspase-1 Activation, Nitric Oxide and Cytokines Production During Experimental Sporotrichosis

Autor(es)

Goncalves, Amanda Costa ^[1] ; Geraldo Maia, Danielle Cardoso ^[1] ; Ferreira, Lucas Souza ^[1] ; Silva Monnazzi, Luis Gustavo ^[1] ; Alegranci, Pamela ^[1] ; Polesi Placeres, Marisa Campos ^[1] ; Batista-Duharte, Alexander ^[1] ; Carlos, Iracilda Zeppone ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Clin Anal, Fac Pharmaceut Sci, BR-14801902 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Mycopathologia; v. 179, n. 1-2, p. 21-30, FEB 2015.
• Citações Web of Science: 16

Resumo

Sporotrichosis is a chronic infection caused by the dimorphic fungus Sporothrix schenckii, involving all layers of skin and the subcutaneous tissue. The role of innate immune toll-like receptors 2 and 4 in the defense against this fungus has been reported, but so far, there were no studies on the effect of cell wall major components over the cytosolic oligo-merization domain (NOD)-like receptors, important regulators of inflammation and responsible for the maturation of IL-1 beta and IL-18, whose functions are dependents of the caspase-1 activation, that can participate of inflammasome. It was evaluated the percentage of activation of caspase-1, the production of IL-1 beta, IL-18, IL-17, IFN-gamma and nitric oxide in a Balb/c model of S. schenckii infection. It was observed a decreased activity of caspase-1 during the fourth and sixth weeks of infection accompanied by reduced secretion of the cytokines IL-1 beta, IL-18 and IL-17 and high production of nitric oxide. IFN-gamma levels were elevated during the entire time course of infection. This temporal reduction in caspase-1 activity coincides exactly with the reported period of fungal burden associated with a transitory immunosuppression induced by this fungus and detected in similar infection models. These results indicate the importance of interaction between caspase-1, cytokines IL-1 beta and IL-18 in the host defense against S. schenckii infection, suggesting a participation the inflammasome in this response. (AU)

• Processo FAPESP: 13/03190-5 - Papel do inflamassoma em infecção fúngica induzida por Sporothrix schenckii
• Beneficiário: Amanda Costa Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Doutorado