A suite of microsatellite markers for genetic management of captive cracids (Aves, Galliformes)

Autor(es)

Costa, M. C. ^[1] ; Camargo, C. ^[1] ; Laganaro, N. M. ^[1] ; Oliveira, Jr., P. R. R. ^[1] ; Davanco, P. V. ^[1] ; Azeredo, R. M. A. ^[2] ; Simpson, J. G. P. ^[2] ; Silveira, L. F. ^[3] ; Francisco, M. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Ciencias Ambientais, Sorocaba, SP - Brazil

^[2] Soc Pesquisa Manejo & Reprod Fauna Silvestre, CRAX, Contagem, MG - Brazil

^[3] Univ Sao Paulo, Museu Zool, Secao Aves, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 13, n. 4, p. 9867-9873, 2014.
• Citações Web of Science: 2

Resumo

Cracids are medium to large frugivorous birds that are endemic to the Neotropics. Because of deforestation and overhunting, many species are threatened. The conservation of several species has relied on captive breeding and reintroduction in the wild, but captive populations may be inbred. Microsatellite tools can permit the construction of genetic pedigrees to reduce inbreeding, but only a few loci are available for this group of birds. Here, we present 10 novel polymorphic microsatellite loci and the cross-amplification of these and of 10 additional loci available in the literature in a panel of 5 cracid species, including 3 species with high conservation concern. We provide the first polymorphic loci for the jacutinga, Aburria jacutinga (N = 8), and red-billed curassow, Crax blumenbachii (N = 9), and additional loci for bare-faced curassow, C. fasciolata (N = 8), Alagoas curassow, Pauxi mitu (N = 5), and razor-billed curassow, P. tuberosa (N = 5). The average number of alleles was 2.9 for A. jacutinga, 2.7 for C. blumenbachii, 3.5 for C. fasciolata, 2.6 for P. mitu, and 5.7 for P. tuberosa. The mean expected heterozygosities were 0.42, 0.40, 0.48, 0.37, and 0.59, respectively. The average probabilities that the set of loci would not exclude a pair of parents of an arbitrary offspring were 2.9% in A. jacutinga, 1% in C. blumenbachii, 0.5% in C. fasciolata, 0.4% in P. mitu, and 0.002% in P. tuberosa suggesting that these loci may be adequate for parentage analysis and to implement ex situ genetic management plans. (AU)

• Processo FAPESP: 10/08586-6 - Isolamento e caracterização de loci de microssatélites para a Jacutinga, Aburria jacutinga (Aves, Cracidae)
• Beneficiário: Crisley de Camargo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 08/51197-0 - Estudos da variabilidade genética de Cracídeos brasileiros ameaçados de extinção (Aves, Galliformes), como subsídios para a conservação ex situ
• Beneficiário: Mercival Roberto Francisco
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/06210-1 - Transferibilidade de loci de microssatélites em aves da família Cracidae (Galliformes).
• Beneficiário: Mariellen Cristine Costa
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica