| Full text | |
| Author(s): |
Costa, M. C.
[1]
;
Camargo, C.
[1]
;
Laganaro, N. M.
[1]
;
Oliveira, Jr., P. R. R.
[1]
;
Davanco, P. V.
[1]
;
Azeredo, R. M. A.
[2]
;
Simpson, J. G. P.
[2]
;
Silveira, L. F.
[3]
;
Francisco, M. R.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Ciencias Ambientais, Sorocaba, SP - Brazil
[2] Soc Pesquisa Manejo & Reprod Fauna Silvestre, CRAX, Contagem, MG - Brazil
[3] Univ Sao Paulo, Museu Zool, Secao Aves, Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Genetics and Molecular Research; v. 13, n. 4, p. 9867-9873, 2014. |
| Web of Science Citations: | 2 |
| Abstract | |
Cracids are medium to large frugivorous birds that are endemic to the Neotropics. Because of deforestation and overhunting, many species are threatened. The conservation of several species has relied on captive breeding and reintroduction in the wild, but captive populations may be inbred. Microsatellite tools can permit the construction of genetic pedigrees to reduce inbreeding, but only a few loci are available for this group of birds. Here, we present 10 novel polymorphic microsatellite loci and the cross-amplification of these and of 10 additional loci available in the literature in a panel of 5 cracid species, including 3 species with high conservation concern. We provide the first polymorphic loci for the jacutinga, Aburria jacutinga (N = 8), and red-billed curassow, Crax blumenbachii (N = 9), and additional loci for bare-faced curassow, C. fasciolata (N = 8), Alagoas curassow, Pauxi mitu (N = 5), and razor-billed curassow, P. tuberosa (N = 5). The average number of alleles was 2.9 for A. jacutinga, 2.7 for C. blumenbachii, 3.5 for C. fasciolata, 2.6 for P. mitu, and 5.7 for P. tuberosa. The mean expected heterozygosities were 0.42, 0.40, 0.48, 0.37, and 0.59, respectively. The average probabilities that the set of loci would not exclude a pair of parents of an arbitrary offspring were 2.9% in A. jacutinga, 1% in C. blumenbachii, 0.5% in C. fasciolata, 0.4% in P. mitu, and 0.002% in P. tuberosa suggesting that these loci may be adequate for parentage analysis and to implement ex situ genetic management plans. (AU) | |