Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone

Autor(es)

Fukumasu, H. ^[1] ; Cordeiro, Y. G. ^[1] ; Rochetti, A. L. ^[1] ; Barra, C. N. ^[1] ; Samora, T. S. ^[1] ; Strefezzi, R. F. ^[1] ; Dagli, M. L. Z. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Vet Med, Lab Oncol Comparada & Translac, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Lab Oncol Expt & Comparada, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 48, n. 3, p. 240-244, MAR 2015.
• Citações Web of Science: 3

Resumo

Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were NR1I3−. Compared with benign lesions, malignant lesions had less NR1I3+ tumor cells. Gene expression analysis also showed an inverse correlation between NR1I3 mRNA expression and tumor size (P=0.0061), suggesting that bigger tumors expressed less NR1I3 transcripts, in accordance with our immunohistochemical NR1I3 tests. Our results indicate that NR1I3 expression decreased during progression of malignant lung tumors induced by NNK in mice. (AU)

• Processo FAPESP: 10/05650-5 - Avaliação nutrigenética do receptor androstano constitutivo (CAR, NR1i3) sobre a eficiência alimentar em bovinos da raça Nelore
• Beneficiário: Heidge Fukumasu
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/11081-6 - Caracterização da expressão e localização do receptor car (nr1i3) em tumores de pulmão induzidos pelo nnk [4-(metilnitrosamino)-1-(3-piridil)-1-butanona] em camundongos
• Beneficiário: Tássia Sant'Ana Samóra
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 08/56584-2 - Avaliação do papel do receptor CAR (constitutive androstane receptor, NR1i3) em tumores de pulmão: ênfase na terapia antitumoral
• Beneficiário: Heidge Fukumasu
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/00535-3 - Treinamento técnico em cultivo de células e biologia molecular
• Beneficiário: Arina Lázaro Rochetti
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico