Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone

Author(s)

Fukumasu, H. ^[1] ; Cordeiro, Y. G. ^[1] ; Rochetti, A. L. ^[1] ; Barra, C. N. ^[1] ; Samora, T. S. ^[1] ; Strefezzi, R. F. ^[1] ; Dagli, M. L. Z. ^[2]

Affiliation

^[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Vet Med, Lab Oncol Comparada & Translac, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Lab Oncol Expt & Comparada, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Medical and Biological Research; v. 48, n. 3, p. 240-244, MAR 2015.
• Web of Science Citations: 3

Abstract

Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were NR1I3−. Compared with benign lesions, malignant lesions had less NR1I3+ tumor cells. Gene expression analysis also showed an inverse correlation between NR1I3 mRNA expression and tumor size (P=0.0061), suggesting that bigger tumors expressed less NR1I3 transcripts, in accordance with our immunohistochemical NR1I3 tests. Our results indicate that NR1I3 expression decreased during progression of malignant lung tumors induced by NNK in mice. (AU)

• FAPESP's process: 10/05650-5 - Nutrigenetic evaluation of constitutive androstane receptor (CAR, NR1i3) on feed efficiency in Nelore cattle
• Grantee: Heidge Fukumasu
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/11081-6 - Characterization of expression and localization of CAR receptor (NR1i3) in NNK [4-(METHYLNITROSAMINE)-1-(3-PIRYDIL)-1-BUTANONE]-induced lung tumors in mice
• Grantee: Tássia Sant'Ana Samóra
• Support Opportunities: Scholarships in Brazil - Scientific Initiation