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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dendritic cell-derived exosomes may be a tool for cancer immunotherapy by converting tumor cells into immunogenic targets

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Autor(es):
Romagnoli, Graziela Gorete [1] ; Zelante, Bruna Barbosa [1] ; Toniolo, Patricia Argenta [1] ; Migliori, Isabella Katz [1] ; Barbuto, Jose Alexandre M. [1, 2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Lab Tumor Immunol, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Ctr Cellular & Mol Studies & Therapy NETCEM, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN IMMUNOLOGY; v. 5, JAN 19 2015.
Citações Web of Science: 41
Resumo

Dendritic cells (DCs) have been attracting attention in cancer immunotherapy because of their role in inducing and modulating effective immune responses. Besides the direct contact with other cell types and the secretion of cytokines, it is becoming clear that nanovesicles, such as exosomes (Exo), secreted by DCs also have a role in their function. Conversely, tumor-derived Exo carry antigens and have been used as a source of specific stimulus for the immune response against tumors. At the same time, several works have shown that different cells types incorporate DC-derived Exo (DC-Exo), resulting in modifications of their phenotype and function. Since DC-Exo carry many of the immune function-associated molecules of DCs, their incorporation by tumor cells could turn tumor cells into immunogenic targets. We have, therefore, treated human breast adenocarcinoma cells (SK-BR-3) with DCs-Exo and used these to stimulate previously SK-BR-3-primed CD3(+) T-cells. Sensitized T-cells cultured with DC-Exo-treated tumor cells showed a significantly higher percentage of IFN-gamma-secreting cells (as measured by ELISPOT), when compared to the frequency of cells responding to non-DC-Exo-treated cells. These data show that the incorporation of DC-Exo by the tumor cells increased their ability to activate T-cells for a possibly more effective response, thus showing that DC-Exo may become another tool in cancer immunotherapy. (AU)

Processo FAPESP: 09/54599-5 - Células dendríticas: elementos integrados do sistema imune - enfoque aplicado
Beneficiário:Jose Alexandre Marzagão Barbuto
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 07/58597-1 - Exossomos derivados de células dendríticas como adjuvantes naturais na resposta antitumoral
Beneficiário:Graziela Gorete Romagnoli
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 04/09956-0 - Modulação funcional de células dentríticas em diferentes situações fisiopatológicas
Beneficiário:Jose Alexandre Marzagão Barbuto
Linha de fomento: Auxílio à Pesquisa - Temático