| Full text | |
| Author(s): |
Romagnoli, Graziela Gorete
[1]
;
Zelante, Bruna Barbosa
[1]
;
Toniolo, Patricia Argenta
[1]
;
Migliori, Isabella Katz
[1]
;
Barbuto, Jose Alexandre M.
[1, 2]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Lab Tumor Immunol, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Ctr Cellular & Mol Studies & Therapy NETCEM, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN IMMUNOLOGY; v. 5, JAN 19 2015. |
| Web of Science Citations: | 41 |
| Abstract | |
Dendritic cells (DCs) have been attracting attention in cancer immunotherapy because of their role in inducing and modulating effective immune responses. Besides the direct contact with other cell types and the secretion of cytokines, it is becoming clear that nanovesicles, such as exosomes (Exo), secreted by DCs also have a role in their function. Conversely, tumor-derived Exo carry antigens and have been used as a source of specific stimulus for the immune response against tumors. At the same time, several works have shown that different cells types incorporate DC-derived Exo (DC-Exo), resulting in modifications of their phenotype and function. Since DC-Exo carry many of the immune function-associated molecules of DCs, their incorporation by tumor cells could turn tumor cells into immunogenic targets. We have, therefore, treated human breast adenocarcinoma cells (SK-BR-3) with DCs-Exo and used these to stimulate previously SK-BR-3-primed CD3(+) T-cells. Sensitized T-cells cultured with DC-Exo-treated tumor cells showed a significantly higher percentage of IFN-gamma-secreting cells (as measured by ELISPOT), when compared to the frequency of cells responding to non-DC-Exo-treated cells. These data show that the incorporation of DC-Exo by the tumor cells increased their ability to activate T-cells for a possibly more effective response, thus showing that DC-Exo may become another tool in cancer immunotherapy. (AU) | |
| FAPESP's process: | 04/09956-0 - Functional modulation of dendritic cells in different pathophysiological conditions |
| Grantee: | Jose Alexandre Marzagão Barbuto |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 09/54599-5 - Dendritic cells: integrative elements of the immune system - an applied approach |
| Grantee: | Jose Alexandre Marzagão Barbuto |
| Support Opportunities: | Research Projects - Thematic Grants |