Quantitative T2 Combined with Texture Analysis of Nuclear Magnetic Resonance Images Identify Different Degrees of Muscle Involvement in Three Mouse Models of Muscle Dystrophy: mdx, Large(myd) and mdx/Large(myd)

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Martins-Bach, Aurea B. ^{[1, 2]} ; Malheiros, Jackeline ^{[3, 4]} ; Matot, Beatrice ^{[2, 5]} ; Martins, Poliana C. M. ^[1] ; Almeida, Camila F. ^[1] ; Caldeira, Waldir ^[6] ; Ribeiro, Alberto F. ^[6] ; de Sousa, Paulo Loureiro ^{[2, 5, 7]} ; Azzabou, Noura ^{[2, 5]} ; Tannsus, Alberto ^[3] ; Carlier, Pierre G. ^{[2, 5]} ; Vainzof, Mariz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, IB, Dept Genet & Biol Evolut, Ctr Estudos Genoma Humano, BR-09500900 Sao Paulo, SP - Brazil

^[2] Inst Myol, NMR Lab, Paris - France

^[3] Univ Sao Paulo, IFSC, Ctr Imagens & Espectroscopia In Vivo Ressonancia, BR-09500900 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Dept Fisiol, Sao Paulo, SP - Brazil

^[5] CEA, I2BM, MIRCen, NMR Lab, Paris - France

^[6] Univ Sao Paulo, IB, Dept Genet & Biol Evolut, Lab Microscopia Eletron, BR-09500900 Sao Paulo, SP - Brazil

^[7] Univ Strasbourg, CNRS, ICube UMR 7357, FMTS, Strasbourg - France

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 2 FEB 24 2015.
• Citações Web of Science: 5

Resumo

Quantitative nuclear magnetic resonance imaging (MRI) has been considered a promising non-invasive tool for monitoring therapeutic essays in small size mouse models of muscular dystrophies. Here, we combined MRI (anatomical images and transverse relaxation time constant-T2-measurements) to texture analyses in the study of four mouse strains covering a wide range of dystrophic phenotypes. Two still unexplored mouse models of muscular dystrophies were analyzed: The severely affected Large(myd) mouse and the recently generated and worst double mutant mdx/Large(myd) mouse, as compared to the mildly affected mdx and normal mice. The results were compared to histopathological findings. MRI showed increased intermuscular fat and higher muscle T2 in the three dystrophic mouse models when compared to the wild-type mice (T2: mdx/Large(myd): 37.6 +/- 2.8 ms; mdx: 35.2 +/- 4.5 ms; Large(myd): 36.6 +/- 4.0 ms; wild-type: 29.1 +/- 1.8 ms, p<0.05), in addition to higher muscle T2 in the mdx/Large(myd) mice when compared to mdx (p<0.05). The areas with increased muscle T2 in the MRI correlated spatially with the identified histopathological alterations such as necrosis, inflammation, degeneration and regeneration foci. Nevertheless, muscle T2 values were not correlated with the severity of the phenotype in the 3 dystrophic mouse strains, since the severely affected Large(myd) showed similar values than both the mild mdx and worst mdx/Large(myd) lineages. On the other hand, all studied mouse strains could be unambiguously identified with texture analysis, which reflected the observed differences in the distribution of signals in muscle MRI. Thus, combined T2 intensity maps and texture analysis is a powerful approach for the characterization and differentiation of dystrophic muscles with diverse genotypes and phenotypes. These new findings provide important noninvasive tools in the evaluation of the efficacy of new therapies, and most importantly, can be directly applied in human translational research. (AU)

• Processo FAPESP: 98/14254-2 - Centro de Estudos do Genoma Humano
• Beneficiário: Mayana Zatz
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/08028-1 - CEGH-CEL - Centro de Estudos do Genoma Humano e de Células-Tronco
• Beneficiário: Mayana Zatz
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs