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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Vagotomy ameliorates islet morphofunction and body metabolic homeostasis in MSG-obese rats

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Autor(es):
Lubaczeuski, C. [1] ; Balbo, S. L. [1] ; Ribeiro, R. A. [2] ; Vettorazzi, J. F. [3] ; Santos-Silva, J. C. [3] ; Carneiro, E. M. [3] ; Bonfleur, M. L. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Oeste Parana, Ctr Ciencias Biol & Saude, Lab Fisiol Endocrina & Metab, Cascavel, PR - Brazil
[2] Univ Fed Rio de Janeiro, Macae, RJ - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biol Estrutural & Func, Lab Pancreas Endocrino & Metab, Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Medical and Biological Research; v. 48, n. 5, p. 447-457, MAY 2015.
Citações Web of Science: 10
Resumo

The parasympathetic nervous system is important for beta-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic beta-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline {[}control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon carbachol (Cch) stimulus, despite a higher intracellular Ca2+ mobilization. Furthermore, while the pancreas weight was 34% lower in MSG rats, no alteration in islet and beta-cell mass was observed. However, in the MSG pancreas, increases of 51% and 55% were observed in the total islet and beta-cell area/pancreas section, respectively. Also, the beta-cell number per beta-cell area was 19% higher in MSG rat pancreas than in CTL pancreas. Vagotomy prevented obesity, reducing 25% of body fat stores and ameliorated glucose homeostasis in Mvag rats. Mvag islets demonstrated partially reduced insulin secretion in response to 11.1 mM glucose and presented normalization of Cch-induced Ca2+ mobilization and insulin release. All morphometric parameters were similar among Mvag and CTL rat pancreases. Therefore, the higher insulin release in MSG rats was associated with greater beta-cell/islet numbers and not due to hypertrophy. Vagotomy improved whole body nutrient homeostasis and endocrine pancreatic morphofunction in Mvag rats. (AU)

Processo FAPESP: 11/09012-6 - Mecanismos moleculares envolvidos na disfunção e morte de células beta pancreáticas no Diabetes Mellitus: estratégias para a inibição desses processos e para a recuperação da massa insular em diferentes modelos celulares e animais
Beneficiário:Antonio Carlos Boschiero
Linha de fomento: Auxílio à Pesquisa - Temático