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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Vagotomy ameliorates islet morphofunction and body metabolic homeostasis in MSG-obese rats

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Author(s):
Lubaczeuski, C. [1] ; Balbo, S. L. [1] ; Ribeiro, R. A. [2] ; Vettorazzi, J. F. [3] ; Santos-Silva, J. C. [3] ; Carneiro, E. M. [3] ; Bonfleur, M. L. [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Oeste Parana, Ctr Ciencias Biol & Saude, Lab Fisiol Endocrina & Metab, Cascavel, PR - Brazil
[2] Univ Fed Rio de Janeiro, Macae, RJ - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biol Estrutural & Func, Lab Pancreas Endocrino & Metab, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 48, n. 5, p. 447-457, MAY 2015.
Web of Science Citations: 10
Abstract

The parasympathetic nervous system is important for beta-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic beta-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline {[}control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon carbachol (Cch) stimulus, despite a higher intracellular Ca2+ mobilization. Furthermore, while the pancreas weight was 34% lower in MSG rats, no alteration in islet and beta-cell mass was observed. However, in the MSG pancreas, increases of 51% and 55% were observed in the total islet and beta-cell area/pancreas section, respectively. Also, the beta-cell number per beta-cell area was 19% higher in MSG rat pancreas than in CTL pancreas. Vagotomy prevented obesity, reducing 25% of body fat stores and ameliorated glucose homeostasis in Mvag rats. Mvag islets demonstrated partially reduced insulin secretion in response to 11.1 mM glucose and presented normalization of Cch-induced Ca2+ mobilization and insulin release. All morphometric parameters were similar among Mvag and CTL rat pancreases. Therefore, the higher insulin release in MSG rats was associated with greater beta-cell/islet numbers and not due to hypertrophy. Vagotomy improved whole body nutrient homeostasis and endocrine pancreatic morphofunction in Mvag rats. (AU)

FAPESP's process: 11/09012-6 - Molecular mechanisms involved in the dysfunction and death of pancreatic beta cells in Diabetes Mellitus: strategies for the prevention of islet dysfunction and for islet mass recuperation in different cellular and animal models
Grantee:Antonio Carlos Boschiero
Support type: Research Projects - Thematic Grants