T-3 rapidly regulates several steps of alpha subunit glycoprotein (CGA) synthesis and secretion in the pituitary of male rats: Potential repercussions on TSH, FSH and LH secretion

Autor(es)

Bargi-Souza, Paula ^[1] ; Romano, Renata Marino ^[1] ; Goulart-Silva, Francemilson ^[1] ; Brunetto, Erika Lia ^[1] ; Nunes, Maria Tereza ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 409, n. C, p. 73-81, JUL 5 2015.
• Citações Web of Science: 11

Resumo

TSH, FSH and LH share the same glycoprotein alpha chain (CGA) as part of their protein structure. Therefore, it is possible that thyroid and gonadal dysfunction may affect the CGA expression. This study evaluated several steps of CGA synthesis and secretion in thyrotrophs and gonadotrophs of control and hypothyroid rats, acutely or chronically-treated with T-3. Hypothyroidism increased the Cga mRNA expression and its association to ribosome, but decreased intracellular CGA content. These parameters were reversed after acute or chronic T-3 treatment. We conclude that T-3 not only down-regulates Cga mRNA expression, as expected, but also inhibits the association of Cga mRNA to ribosome, as well as the CGA secretion. These findings add novel insights into our understanding of the role of T-3 on the regulation of the Cga gene expression and CGA secretion, which might have a potential repercussion in all pituitary glycoprotein hormone synthesis and secretion. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/56631-8 - Efeito da administracao aguda de triiodotironina (t3) sobre a regulacao pos-transcricional da expressao genica do hormonio tireotrofico (beta-tsh).
• Beneficiário: Paula Bargi de Souza
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 06/59863-4 - Acao nao genomica dos hormonios tireoideanos sobre o musculo cardiaco: repercusoes sobre o metabolismo energetico.
• Beneficiário: Erika Lia Brunetto Ract
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/50977-2 - Influencia do hormonio tiroideano na atividade do gonadotrofo.
• Beneficiário: Renata Marino Romano
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/61713-0 - Acoes nao genomicas dos hormonios tireoideanos na hipofise de ratos: repercussoes na sintese e secrecao de hormonio do crescimento (gh) e tireotrofico (tsh).
• Beneficiário: Maria Tereza Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular