| Texto completo | |
| Autor(es): |
Silva, Adriana Farias
[1]
;
Silva, Leandro de Souza
[2]
;
Alves, Flavio Lopes
[3]
;
TorossianTorres, Marcelo Der
[1]
;
de SaPinheiro, Ana Acacia
[2]
;
Miranda, Antonio
;
LaraCapurro, Margareth
[4]
;
Oliveira, Jr., Vani Xavier
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Fed ABC, Cre Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21941 Rio De Janeiro, RJ - Brazil
[3] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Inst Ciencias Biomed II, Dept Parasitol, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Experimental Parasitology; v. 153, p. 1-7, JUN 2015. |
| Citações Web of Science: | 2 |
| Resumo | |
The anti-plasmodium activity of angiotensin II and its analogs have been described in different plasmodium species. Here we synthesized angiotensin II Ala-scan analogs to verify peptide-parasite invasion preservation with residue replacements. The analogs were synthesized by 9-fluorenylmethoxycarbonyl (Fmoc) and tert-butyloxycarbonyl (t-Boc) solid phase methods, purified by liquid chromatography and characterized by mass spectrometry. The results obtained in Plasmodium falciparum assays indicated that all analogs presented some influence in parasite invasion, except {[}Ala(4)]-Ang II (18% of anti-plasmodium activity) that was not statistically different from control. Although {[}Ala(8)]-Ang II presented a lower biological activity (20%), it was statistically different from control. The most relevant finding was that {[}Ala(5)]-Ang II preserved activity (45%) relative to Ang II (47%). In the results of Plasmodium gallinaceum assays all analogs were not statistically different from control, except {[}Ala(6)]-Ang II, which was able to reduce the parasitemia about 49%. This approach provides insight for understanding the importance of each amino acid on the native Ang II sequence and provides a new direction for the design of potential chemotherapeutic agents without pressor activity. (C) 2015 Elsevier Inc. All rights reserved. (AU) | |
| Processo FAPESP: | 11/11348-2 - Peptídeos Antimaláricos Derivados da Angiotensina II |
| Beneficiário: | Adriana Farias da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 11/10823-9 - Compostos antimaláricos derivados da angiotensina II |
| Beneficiário: | Vani Xavier de Oliveira Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/12938-6 - Peptídeos biologicamente ativos em micro-organismos patogênicos |
| Beneficiário: | Vani Xavier de Oliveira Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |