Antiplasmodial angiotensin II derivatives studies in vivo & in vitro
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| Full text | |
| Author(s): |
Silva, Adriana Farias
[1]
;
Silva, Leandro de Souza
[2]
;
Alves, Flavio Lopes
[3]
;
TorossianTorres, Marcelo Der
[1]
;
de SaPinheiro, Ana Acacia
[2]
;
Miranda, Antonio
;
LaraCapurro, Margareth
[4]
;
Oliveira, Jr., Vani Xavier
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed ABC, Cre Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21941 Rio De Janeiro, RJ - Brazil
[3] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Inst Ciencias Biomed II, Dept Parasitol, Sao Paulo, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Experimental Parasitology; v. 153, p. 1-7, JUN 2015. |
| Web of Science Citations: | 2 |
| Abstract | |
The anti-plasmodium activity of angiotensin II and its analogs have been described in different plasmodium species. Here we synthesized angiotensin II Ala-scan analogs to verify peptide-parasite invasion preservation with residue replacements. The analogs were synthesized by 9-fluorenylmethoxycarbonyl (Fmoc) and tert-butyloxycarbonyl (t-Boc) solid phase methods, purified by liquid chromatography and characterized by mass spectrometry. The results obtained in Plasmodium falciparum assays indicated that all analogs presented some influence in parasite invasion, except {[}Ala(4)]-Ang II (18% of anti-plasmodium activity) that was not statistically different from control. Although {[}Ala(8)]-Ang II presented a lower biological activity (20%), it was statistically different from control. The most relevant finding was that {[}Ala(5)]-Ang II preserved activity (45%) relative to Ang II (47%). In the results of Plasmodium gallinaceum assays all analogs were not statistically different from control, except {[}Ala(6)]-Ang II, which was able to reduce the parasitemia about 49%. This approach provides insight for understanding the importance of each amino acid on the native Ang II sequence and provides a new direction for the design of potential chemotherapeutic agents without pressor activity. (C) 2015 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 11/11348-2 - Antimalarial Peptides derivative from Angiotensin II |
| Grantee: | Adriana Farias da Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 11/10823-9 - Antimalarial compounds derivative from angiotensin II |
| Grantee: | Vani Xavier de Oliveira Junior |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/12938-6 - Biologically active peptides against pathogenic micro-organisms |
| Grantee: | Vani Xavier de Oliveira Junior |
| Support Opportunities: | Regular Research Grants |