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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of the angiotensin II Ala-scan analogs in erythrocytic cycle of Plasmodium falciparum (in vitro) and Plasmodium gallinaceum (ex vivo)

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Author(s):
Silva, Adriana Farias [1] ; Silva, Leandro de Souza [2] ; Alves, Flavio Lopes [3] ; TorossianTorres, Marcelo Der [1] ; de SaPinheiro, Ana Acacia [2] ; Miranda, Antonio ; LaraCapurro, Margareth [4] ; Oliveira, Jr., Vani Xavier [1]
Total Authors: 8
Affiliation:
[1] Univ Fed ABC, Cre Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21941 Rio De Janeiro, RJ - Brazil
[3] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Inst Ciencias Biomed II, Dept Parasitol, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Experimental Parasitology; v. 153, p. 1-7, JUN 2015.
Web of Science Citations: 2
Abstract

The anti-plasmodium activity of angiotensin II and its analogs have been described in different plasmodium species. Here we synthesized angiotensin II Ala-scan analogs to verify peptide-parasite invasion preservation with residue replacements. The analogs were synthesized by 9-fluorenylmethoxycarbonyl (Fmoc) and tert-butyloxycarbonyl (t-Boc) solid phase methods, purified by liquid chromatography and characterized by mass spectrometry. The results obtained in Plasmodium falciparum assays indicated that all analogs presented some influence in parasite invasion, except {[}Ala(4)]-Ang II (18% of anti-plasmodium activity) that was not statistically different from control. Although {[}Ala(8)]-Ang II presented a lower biological activity (20%), it was statistically different from control. The most relevant finding was that {[}Ala(5)]-Ang II preserved activity (45%) relative to Ang II (47%). In the results of Plasmodium gallinaceum assays all analogs were not statistically different from control, except {[}Ala(6)]-Ang II, which was able to reduce the parasitemia about 49%. This approach provides insight for understanding the importance of each amino acid on the native Ang II sequence and provides a new direction for the design of potential chemotherapeutic agents without pressor activity. (C) 2015 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/11348-2 - Antimalarial Peptides derivative from Angiotensin II
Grantee:Adriana Farias da Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/12938-6 - Biologically active peptides against pathogenic micro-organisms
Grantee:Vani Xavier de Oliveira Junior
Support type: Regular Research Grants
FAPESP's process: 11/10823-9 - Antimalarial compounds derivative from angiotensin II
Grantee:Vani Xavier de Oliveira Junior
Support type: Regular Research Grants