Effects of intermittent fasting on age-related changes on Na,K-ATPase activity and oxidative status induced by lipopolysaccharide in rat hippocampus

Autor(es):Mostrar menos -

Vasconcelos, Andrea Rodrigues ^[1] ; Kinoshita, Paula Fernanda ^[1] ; Yshii, Lidia Mitiko ^[1] ; Marques Orellana, Ana Maria ^[1] ; Boehmer, Ana Elisa ^[1] ; Lima, Larissa de Sa ^[1] ; Alves, Rosana ^[1] ; Andreotti, Diana Zukas ^[1] ; Marcourakis, Tania ^[2] ; Scavone, Cristoforo ^[1] ; Kawamoto, Elisa Mitiko ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Mol Neuropharmacol Lab, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NEUROBIOLOGY OF AGING; v. 36, n. 5, p. 1914-1923, MAY 2015.
• Citações Web of Science: 12

Resumo

Chronic neuroinflammation is a common characteristic of neurodegenerative diseases, and lipopolysaccharide (LPS) signaling is linked to glutamate-nitric oxide-Na,K-ATPase isoforms pathway in central nervous system (CNS) and also causes neuroinflammation. Intermittent fasting (IF) induces adaptive responses in the brain that can suppress inflammation, but the age-related effect of IF on LPS modulatory influence on nitric oxide-Na,K-ATPase isoforms is unknown. This work compared the effects of LPS on the activity of alpha(1), alpha(2,3) Na,K-ATPase, nitric oxide synthase gene expression and/or activity, cyclic guanosine monophosphate, 3-nitrotyrosineecontaining proteins, and levels of thiobarbituric acidereactive substances in CNS of young and older rats submitted to the IF protocol for 30 days. LPS induced an agerelated effect in neuronal nitric oxide synthase activity, cyclic guanosine monophosphate, and levels of thiobarbituric acidereactive substances in rat hippocampus that was linked to changes in a2,3-Na,KATPase activity, 3-nitrotyrosine proteins, and inducible nitric oxide synthase gene expression. IF induced adaptative cellular stress-response signaling pathways reverting LPS effects in rat hippocampus of young and older rats. The results suggest that IF in both ages would reduce the risk for deficits on brain function and neurodegenerative disorders linked to inflammatory response in the CNS. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/10303-5 - Investigação do papel de TRAF6 na interação com alfa-sinucleína e na consequente modulação em vias de sinalização
• Beneficiário: Lidia Mitiko Yshii
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado