Tissue hyaluronan expression, as reflected in the sputum of lung cancer patients, is an indicator of malignancy

Autor(es):Mostrar menos -

Rangel, M. P. ^[1] ; de Sa, V. K. ^[1] ; Martins, V. ^[1] ; Martins, J. R. M. ^{[2, 3]} ; Parra, E. R. ^[1] ; Mendes, A. ^[2] ; Andrade, P. C. ^[1] ; Reis, R. M. ^{[4, 5, 6]} ; Longatto-Filho, A. ^{[4, 5, 6, 7]} ; Oliveira, C. Z. ^[6] ; Takagaki, T. ^[8] ; Carraro, D. M. ^[9] ; Nader, H. B. ^[2] ; Capelozzi, V. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Patol, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Fac Med, Dept Bioquim, Disciplina Biol Mol, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Med, Disciplina Endocrinol & Metab, LEMT, Sao Paulo, SP - Brazil

^[4] Univ Minho, Sch Hlth Sci, Life & Hlth Sci Res Inst ICVS, Braga - Portugal

^[5] PT Govt Associate Lab, ICVS 3Bs, Guimaraes - Portugal

^[6] Fundacao Pio XII, Hosp Canc Barretos, Ctr Pesquisa Oncol Mol, Barretos, SP - Brazil

^[7] Univ Sao Paulo, Fac Med, LIM 14, Sao Paulo, SP - Brazil

^[8] Univ Sao Paulo, Fac Med, Inst Coracao, Div Pneumol, Sao Paulo, SP - Brazil

^[9] AC Camargo Canc Ctr, Ctr Int Pesquisa CIPE, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 48, n. 6, p. 557-567, JUN 2015.
• Citações Web of Science: 6

Resumo

Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral stroma. Similarly significant was the direct association between N1 tumors and high levels of HA in cancer cells. Cox multivariate analysis showed significant association between better survival and low HA. HA increased in sputum from lung cancer patients compared to cancer-free and healthy volunteers and a significant correlation was found between HA in sputum and HA in cancer tissue. Localization of HA in tumor tissue was related to malignancy and reflected in sputum, making this an emerging factor for an important diagnostic procedure in patients suspected to have lung cancer. Further study in additional patients in a randomized prospective trial is required to finalize these results and to validate our quantitative assessment of HA, as well as to couple it to gold standard sputum cytology. (AU)

• Processo FAPESP: 10/11005-5 - Detecção de ácido hialurônico e expressão gênica de citocinas: impacto preditivo e prognóstico como novos marcadores em câncer de pulmão
• Beneficiário: Vera Luiza Capelozzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular