Combined nitric oxide-releasing poly(vinyl alcohol) film/F127 hydrogel for accelerating wound healing

Autor(es)

Schanuel, Fernanda Seabra ^[1] ; Raggio Santos, Karen Slis ^[2] ; Monte-Alto-Costa, Andrea ^[1] ; de Oliveira, Marcelo G. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estado Rio De Janeiro, UERJ, Dept Histol & Embryol, BR-20950003 Rio De Janeiro - Brazil

^[2] Univ Estadual Campinas, Univ Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 130, p. 182-191, JUN 1 2015.
• Citações Web of Science: 31

Resumo

Nitric oxide (NO) releasing biomaterials represent a potential strategy for use as active wound dressings capable of accelerating wound healing. Topical NO-releasing poly(vinyl alcohol) (PVA) films and Pluronic F127 hydrogels (F127) have already exhibited effective skin vasodilation and wound healing actions. In this study, we functionalized PVA films with SNO groups via esterification with a mixture of mercaptosucinic acid (MSA) and thiolactic acid (TLA) followed by S-nitrosation of the -SH moieties. These films were combined with an underlying layer of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), i.e., PEO-PPO-PEO (Pluronic F127) hydrogel and used for the topical treatment of skin lesions in an animal model. The mixed esterification of PVA with MSA and TLA led to chemically crosslinked PVA-SNO films with a high swelling capacity capable of spontaneously releasing NO. Real time NO-release measurements revealed that the hydrogel layer reduces the initial NO burst from the PVA-SNO films. We demonstrate that the combination of PVA-SNO films with F127 hydrogel accelerates wound contraction, decreases wound gap and cellular density and accelerates the inflammatory phase of the lesion. These results were reflected in an increase in myofibroblastic differentiation and collagen type III expression in the cicatricial tissue. Therefore, PVA-SNO films combined with F127 hydrogel may represent a new approach for active wound dressings capable of accelerating wound healing. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/21936-4 - Filmes de poli(álcool vinílico) funcionalizados com grupos SNO para a o tratamento tópico de lesões
• Beneficiário: Karen Slis Raggio Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 08/57560-0 - Efeitos do chá verde (Camellia sinensis), do cacau e de um doador de óxido nítrico na nefropatia e retinopatia diabética: papel da redução do estresse oxidativo e da inflamação e do aumento do óxido nítrico
• Beneficiário: Jose Butori Lopes de Faria
• Modalidade de apoio: Auxílio à Pesquisa - Temático